Human BHMT ELISA Kit

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  • Alternative name

    Human BHMT1 ELISA Kit;Human HEL-S-61p ELISA Kit;Human betaine--homocysteine S-methyltransferase ELISA Kit;Human betaine--homocysteine S-methyltransferase 1 ELISA Kit;Human epididymis secretory sperm binding protein Li 61p ELISA Kit;

  • Catalog
    E004912
  • species
    Human
  • GeneBHMT
  • Standard CurveHuman BHMT ELISA Kit
  • Other Species Mouse Bhmt ELISA KitBovine BHMT ELISA KitPorcine BHMT ELISA KitRat Bhmt ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Betaine Homocysteine Methyltransferase (BHMT). No significant cross-reactivity or interference between Betaine Homocysteine Methyltransferase (BHMT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.124ng/mL.
  • Intended UseHuman BHMT ELISA Kit allows for the in vitro quantitative determination of BHMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Betaine--homocysteine S-methyltransferase 1 Protein information
  • Uniprot ID BHMT1_HUMAN
  • Uniprot AC Q93088; Q9UNI9;
  • UniGene Hs.80756;
  • GeneID 635
  • KEGG hsa:635;
  • Human Betaine--homocysteine S-methyltransferase 1 Protein SEQUENCE
  • SEQUENCE 406 AA; 44998 MW; 557855B8CEDD0D54 CRC64;

    MPPVGGKKAK KGILERLNAG EIVIGDGGFV FALEKRGYVK AGPWTPEAAV

    EHPEAVRQLH REFLRAGSNV MQTFTFYASE DKLENRGNYV LEKISGQEVN

    EAACDIARQV ADEGDALVAG GVSQTPSYLS CKSETEVKKV FLQQLEVFMK

    KNVDFLIAEY FEHVEEAVWA VETLIASGKP VAATMCIGPE GDLHGVPPGE

    CAVRLVKAGA SIIGVNCHFD PTISLKTVKL MKEGLEAARL KAHLMSQPLA

    YHTPDCNKQG FIDLPEFPFG LEPRVATRWD IQKYAREAYN LGVRYIGGCC

    GFEPYHIRAI AEELAPERGF LPPASEKHGS WGSGLDMHTK PWVRARARKE

    YWENLRIASG RPYNPSMSKP DGWGVTKGTA ELMQQKEATT EQQLKELFEK

    QKFKSQ



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