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Human BTC ELISA Kit

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  • Alternative name

    Human BTC ELISA Kit;Human betacellulin ELISA Kit;Human probetacellulin ELISA Kit;
  • Catalog
    E004910
  • species
    Human
  • GeneBTC
  • Standard CurveHuman BTC ELISA Kit
  • Other Species Mouse Btc ELISA KitBovine BTC ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Betacellulin (bTC). No significant cross-reactivity or interference between Betacellulin (bTC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman BTC ELISA Kit allows for the in vitro quantitative determination of BTC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betacellulin (bTC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betacellulin (bTC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betacellulin (bTC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Betacellulin (bTC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Betacellulin Protein information
  • Uniprot ID BTC_HUMAN
  • Uniprot AC P35070; Q96F48;
  • UniGene Hs.591704; Hs.710156;
  • GeneID 685
  • KEGG hsa:685;
  • Human Betacellulin Protein SEQUENCE

  • SEQUENCE 178 AA; 19746 MW; 27AC77BD92001F0F CRC64;

    MDRAARCSGA SSLPLLLALA LGLVILHCVV ADGNSTRSPE TNGLLCGDPE

    ENCAATTTQS KRKGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYI

    GARCERVDLF YLRGDRGQIL VICLIAVMVV FIILVIGVCT CCHPLRKRRK

    RKKKEEEMET LGKDITPINE DIEETNIA

  • UCSC uc003hig.3; human.;


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