Human ATF4 ELISA Kit

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  • Alternative name

    Human cAMP-dependent transcription factor ATF-4 ELISA Kit;Human activating transcription factor 4 ELISA Kit;Human cyclic AMP-responsive element-binding protein 2 ELISA Kit;Human CREB-2 ELISA Kit;Human cAMP-responsive element-binding protein 2 ELISA Kit;Human DNA-binding protein TAXREB67 ELISA Kit;Human tax-responsive enhancer element-binding protein 67 ELISA Kit;Human TAXREB67 ELISA Kit;Human CREB2 ELISA Kit;Human TXREB ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-4 ELISA Kit;Human cAMP response element-binding protein 2 ELISA Kit;Human tax-responsive enhancer element B67 ELISA Kit;

  • Catalog
    E000491
  • species
    Human
  • GeneATF4
  • Other Species Mouse Atf4 ELISA KitBovine ATF4 ELISA KitRat Atf4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATF4 ELISA Kit allows for the in vitro quantitative determination of ATF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This ATF4/CREB2/TXREB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF4/CREB2/TXREB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY ATF4/CREB2/TXREB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF4/CREB2/TXREB antibody and an ATF4/CREB2/TXREB-HRP conjugate. The assay sample and buffer are incubated together with ATF4/CREB2/TXREB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF4/CREB2/TXREB concentration since ATF4/CREB2/TXREB from samples and ATF4/CREB2/TXREB-HRP conjugate compete for the anti-ATF4/CREB2/TXREB antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF4/CREB2/TXREB from the sample, fewer sites are left to bind ATF4/CREB2/TXREB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF4/CREB2/TXREB concentration in each sample is interpolated from this standard curve.
  • Human Cyclic AMP-dependent transcription factor ATF-4 Protein information
  • Uniprot ID ATF4_HUMAN
  • Uniprot AC P18848; Q9UH31;
  • UniGene Hs.496487;
  • GeneID 468
  • KEGG hsa:468;
  • Human Cyclic AMP-dependent transcription factor ATF-4 Protein SEQUENCE
  • SEQUENCE 351 AA; 38590 MW; 3BBB7379DC3B0D07 CRC64;

    MTEMSFLSSE VLVGDLMSPF DQSGLGAEES LGLLDDYLEV AKHFKPHGFS

    SDKAKAGSSE WLAVDGLVSP SNNSKEDAFS GTDWMLEKMD LKEFDLDALL

    GIDDLETMPD DLLTTLDDTC DLFAPLVQET NKQPPQTVNP IGHLPESLTK

    PDQVAPFTFL QPLPLSPGVL SSTPDHSFSL ELGSEVDITE GDRKPDYTAY

    VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG SPNRSLPSPG

    VLCGSARPKP YDPPGEKMVA AKVKGEKLDK KLKKMEQNKT AATRYRQKKR

    AEQEALTGEC KELEKKNEAL KERADSLAKE IQYLKDLIEE VRKARGKKRV

    P



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