Human ATF4 ELISA Kit

Alternative name
Human cAMP-dependent transcription factor ATF-4 ELISA Kit;Human activating transcription factor 4 ELISA Kit;Human cyclic AMP-responsive element-binding protein 2 ELISA Kit;Human CREB-2 ELISA Kit;Human cAMP-responsive element-binding protein 2 ELISA Kit;Human DNA-binding protein TAXREB67 ELISA Kit;Human tax-responsive enhancer element-binding protein 67 ELISA Kit;Human TAXREB67 ELISA Kit;Human CREB2 ELISA Kit;Human TXREB ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-4 ELISA Kit;Human cAMP response element-binding protein 2 ELISA Kit;Human tax-responsive enhancer element B67 ELISA Kit;
Catalog
E000491
species
Human
GeneATF4
Other Species Mouse Atf4 ELISA Kit Bovine ATF4 ELISA Kit Rat Atf4 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ATF4 ELISA Kit allows for the in vitro quantitative determination of ATF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This ATF4/CREB2/TXREB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF4/CREB2/TXREB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY ATF4/CREB2/TXREB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF4/CREB2/TXREB antibody and an ATF4/CREB2/TXREB-HRP conjugate. The assay sample and buffer are incubated together with ATF4/CREB2/TXREB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF4/CREB2/TXREB concentration since ATF4/CREB2/TXREB from samples and ATF4/CREB2/TXREB-HRP conjugate compete for the anti-ATF4/CREB2/TXREB antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF4/CREB2/TXREB from the sample, fewer sites are left to bind ATF4/CREB2/TXREB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF4/CREB2/TXREB concentration in each sample is interpolated from this standard curve.
Human Cyclic AMP-dependent transcription factor ATF-4 Protein information
Uniprot ID ATF4_HUMAN
Uniprot AC P18848; Q9UH31;
UniGene Hs.496487;
GeneID 468
KEGG hsa:468;
Human Cyclic AMP-dependent transcription factor ATF-4 Protein SEQUENCE
SEQUENCE 351 AA; 38590 MW; 3BBB7379DC3B0D07 CRC64;
MTEMSFLSSE VLVGDLMSPF DQSGLGAEES LGLLDDYLEV AKHFKPHGFS
SDKAKAGSSE WLAVDGLVSP SNNSKEDAFS GTDWMLEKMD LKEFDLDALL
GIDDLETMPD DLLTTLDDTC DLFAPLVQET NKQPPQTVNP IGHLPESLTK
PDQVAPFTFL QPLPLSPGVL SSTPDHSFSL ELGSEVDITE GDRKPDYTAY
VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG SPNRSLPSPG
VLCGSARPKP YDPPGEKMVA AKVKGEKLDK KLKKMEQNKT AATRYRQKKR
AEQEALTGEC KELEKKNEAL KERADSLAKE IQYLKDLIEE VRKARGKKRV
P

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Human ATF4 ELISA Kit