Mouse Col10a1 ELISA Kit

Alternative name
Mouse Col10 ELISA Kit;Mouse Col10a-1 ELISA Kit;Mouse collagen, type X, alpha 1 ELISA Kit;Mouse collagen alpha-1(X) chain ELISA Kit;Mouse procollagen, type X, alpha 1 ELISA Kit;
Catalog
E049057
species
Mouse
GeneCol10a1
Standard Curve
Other Species Bovine COL10A1 ELISA Kit Chicken COL10A1 ELISA Kit Human COL10A1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of COL10A1. No significant cross-reactivity or interference between COL10A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COL10A1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Col10a1 ELISA Kit allows for the in vitro quantitative determination of Col10a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: COL10A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COL10A1 antibody and an COL10A1-HRP conjugate. The assay sample and buffer are incubated together with COL10A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL10A1 concentration since COL10A1 from samples and COL10A1-HRP conjugate compete for the anti-COL10A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL10A1 from the sample, fewer sites are left to bind COL10A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL10A1 concentration in each sample is interpolated from this standard curve.
Mouse Collagen alpha-1(X) chain Protein information
Uniprot ID COAA1_MOUSE
Uniprot AC Q05306;
UniGene Mm.443177;
GeneID 12813
KEGG mmu:12813;
Mouse Collagen alpha-1(X) chain Protein SEQUENCE
SEQUENCE 680 AA; 66775 MW; FE984CA99AF708E2 CRC64;
MLPQIPFLLL MFLTLVHGMF YAERYQTPTG IKGPLASPKT QYFIPYAIKS
KGIPVRGEQG IPGPPGPTGP RGHPGPSGPP GKPGYGSPGL QGEPGLPGPP
GISATGKPGL PGPPGKPGER GPYGHKGDIG PAGLPGPRGP PGPPGIPGPA
GISVPGKPGQ QGLTGAPGPR GFPGEKGAQG APGVNGRKGE TGYGSPGRPG
ERGLPGPQGP IGPPGPSGVG RRGENGFPGQ PGIKGDRGFP GEMGPSGPPG
PQGPPGKQGR EGIGKPGAIG SPGQPGIPGE KGHPGAPGIA GPPGAPGFGK
QGLPGLRGQR GPAGLPGAPG AKGERGPAGH PGEPGLPGSP GNMGPQGPKG
IPGNHGIPGA KGEIGLVGPA GPPGARGARG PPGLDGKTGY PGEPGLNGPK
GNPGLPGQKG DPGVGGTPGL RGPVGPVGAK GVPGHNGEAG PRGEPGIPGT
RGPTGPPGVP GFPGSKGDPG NPGAPGPAGI ATKGLNGPTG PPGPPGPRGH
SGEPGLPGPP GPPGPPGQAV MPDGFIKAGQ RPRLSGMPLV SANHGVTGMP
VSAFTVILSK AYPAVGAPIP FDEILYNRQQ HYDPRSGIFT CKIPGIYYFS
YHVHVKGTHV WVGLYKNGTP TMYTYDEYSK GYLDQASGSA IMELTENDQV
WLQLPNAESN GLYSSEYVHS SFSGFLVAPM
UCSC uc011xcr.1; mouse.;

Category

Mouse Col10a1 ELISA Kit

Mouse Col10 ELISA Kit;Mouse Col10a-1 ELISA Kit;Mouse collagen, type X, alpha 1 ELISA Kit;Mouse collagen alpha-1(X) chain ELISA Kit;Mouse procollagen, type X, alpha 1 ELISA Kit;