Mouse Ccdc83 ELISA Kit

Alternative name
Mouse 4930549K11Rik ELISA Kit;Mouse 4930554C01Rik ELISA Kit;Mouse 4932423M01Rik ELISA Kit;Mouse coiled-coil domain containing 83 ELISA Kit;Mouse coiled-coil domain-containing protein 83 ELISA Kit;
Catalog
E049001
species
Mouse
GeneCcdc83
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCDC83. No significant cross-reactivity or interference between CCDC83 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCDC83 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Ccdc83 ELISA Kit allows for the in vitro quantitative determination of Ccdc83 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CCDC83 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCDC83 antibody and an CCDC83-HRP conjugate. The assay sample and buffer are incubated together with CCDC83-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCDC83 concentration since CCDC83 from samples and CCDC83-HRP conjugate compete for the anti-CCDC83 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCDC83 from the sample, fewer sites are left to bind CCDC83-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCDC83 concentration in each sample is interpolated from this standard curve.
Mouse Coiled-coil domain-containing protein 83 Protein information
Uniprot ID CCD83_MOUSE
Uniprot AC Q9D4V3; Q8CEQ7; Q9D4W3;
UniGene Mm.487222;
GeneID 75338
KEGG mmu:75338;
Mouse Coiled-coil domain-containing protein 83 Protein SEQUENCE
SEQUENCE 305 AA; 35642 MW; 56E81BA749CE109E CRC64;
MDSSAKGSKK DAPDGPPKDS KLPVSEALLD YHREIKENAV ERFMFHIKKL
REKNQKYQER NRRLKEEQNW HIKNLIKELK EKNLDEAPIV TREEVEEAMK
EKWEFERQQE ASLKEMRIQI NEAEKLFLEK LSEKEYWEEY KNVGSAQHAQ
LIVSLQNDID TVKENAEKMS EQYKVTLEDE KKRISRETMI QLKQRKEWAT
QHAVRFIDKN NYREIWENDW LKKEGDTASF GNVLSLLDFL PPDLHTAEPC
VSSGQSRGSL RILAPFALQL WSKLSGLLPT LVTPDPVCII WSFGPGQTNS
MAICP
UCSC uc009igy.1; mouse. [Q9D4V3-1];

Category

Mouse Ccdc83 ELISA Kit

Mouse 4930549K11Rik ELISA Kit;Mouse 4930554C01Rik ELISA Kit;Mouse 4932423M01Rik ELISA Kit;Mouse coiled-coil domain containing 83 ELISA Kit;Mouse coiled-coil domain-containing protein 83 ELISA Kit;