Human ATF3 ELISA Kit

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  • Alternative name

    Human cAMP-dependent transcription factor ATF-3 ELISA Kit;Human activating transcription factor 3 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-3 ELISA Kit;

  • Catalog
    E000488
  • species
    Human
  • GeneATF3
  • Other Species Mouse Atf3 ELISA KitBovine ATF3 ELISA KitRat Atf3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATF3. No significant cross-reactivity or interference between ATF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATF3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL
  • Intended UseHuman ATF3 ELISA Kit allows for the in vitro quantitative determination of ATF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This ATF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ATF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF3 antibody and an ATF3-HRP conjugate. The assay sample and buffer are incubated together with ATF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF3 concentration since ATF3 from samples and ATF3-HRP conjugate compete for the anti-ATF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF3 from the sample, fewer sites are left to bind ATF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF3 concentration in each sample is interpolated from this standard curve.
  • Human Cyclic AMP-dependent transcription factor ATF-3 Protein information
  • Uniprot ID ATF3_HUMAN
  • Uniprot AC P18847; Q5VTZ2; Q6ICQ9; Q7Z566; Q8WYM6;
  • UniGene Hs.460;
  • GeneID 467
  • KEGG hsa:467;
  • Human Cyclic AMP-dependent transcription factor ATF-3 Protein SEQUENCE
  • SEQUENCE 181 AA; 20576 MW; EC5D8F065EEE2D9C CRC64;

    MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ

    NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA

    AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR

    PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S

  • UCSC uc001hjf.4; human. [P18847-1];


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