Human ATF3 ELISA Kit

Alternative name
Human cAMP-dependent transcription factor ATF-3 ELISA Kit;Human activating transcription factor 3 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-3 ELISA Kit;
Catalog
E000488
species
Human
GeneATF3
Other Species Mouse Atf3 ELISA Kit Bovine ATF3 ELISA Kit Rat Atf3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATF3. No significant cross-reactivity or interference between ATF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATF3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL
Intended UseHuman ATF3 ELISA Kit allows for the in vitro quantitative determination of ATF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This ATF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ATF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF3 antibody and an ATF3-HRP conjugate. The assay sample and buffer are incubated together with ATF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF3 concentration since ATF3 from samples and ATF3-HRP conjugate compete for the anti-ATF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF3 from the sample, fewer sites are left to bind ATF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF3 concentration in each sample is interpolated from this standard curve.
Human Cyclic AMP-dependent transcription factor ATF-3 Protein information
Uniprot ID ATF3_HUMAN
Uniprot AC P18847; Q5VTZ2; Q6ICQ9; Q7Z566; Q8WYM6;
UniGene Hs.460;
GeneID 467
KEGG hsa:467;
Human Cyclic AMP-dependent transcription factor ATF-3 Protein SEQUENCE
SEQUENCE 181 AA; 20576 MW; EC5D8F065EEE2D9C CRC64;
MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ
NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA
AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR
PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S
UCSC uc001hjf.4; human. [P18847-1];

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Human ATF3 ELISA Kit

Human cAMP-dependent transcription factor ATF-3 ELISA Kit;Human activating transcription factor 3 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-3 ELISA Kit;