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  • Alternative name

    Coagulation factor VIII ELISA KIT; Antihemophilic factor ELISA KIT; AHF ELISA KIT; Procoagulant componentCleaved into the following 4 chains:Factor VIIIa heavy chain, 200 kDa isoform ELISA KIT; Factor VIIIa heavy chain, 92 kDa isoform ELISA KIT; Factor VIII B chain ELISA KIT; Factor VIIIa light chainF8 ELISA KIT; F8C ELISA KIT; AHF ELISA KIT

  • Catalog
  • species
  • GeneFVIII-Ag
  • Other Species Human FVIII-Ag ELISA KitHuman FVIII Ag ELISA KitMouse FVIII Ag ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.06 ng/ml.
  • Intended UseMouse FVIII-Ag ELISA Kit allows for the in vitro quantitative determination of FVIII-Ag , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse FVIII-Ag monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

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