Mouse Cltc ELISA Kit

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  • Alternative name

    Mouse 3110065L21Rik ELISA Kit;Mouse CHC ELISA Kit;Mouse R74732 ELISA Kit;Mouse clathrin, heavy polypeptide (Hc) ELISA Kit;Mouse clathrin heavy chain 1 ELISA Kit;

  • Catalog
    E048579
  • species
    Mouse
  • GeneCltc
  • Standard CurveMouse Cltc ELISA Kit
  • Other Species Human CLTC ELISA KitBovine CLTC ELISA KitRat Cltc ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CLTC. No significant cross-reactivity or interference between CLTC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLTC and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Cltc ELISA Kit allows for the in vitro quantitative determination of Cltc , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This CLTC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLTC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CLTC ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CLTC antibody and an CLTC-HRP conjugate. The assay sample and buffer are incubated together with CLTC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLTC concentration since CLTC from samples and CLTC-HRP conjugate compete for the anti-CLTC antibody binding site. Since the number of sites is limited, as more sites are occupied by CLTC from the sample, fewer sites are left to bind CLTC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLTC concentration in each sample is interpolated from this standard curve.
  • Mouse Clathrin heavy chain 1 Protein information
  • Uniprot ID CLH1_MOUSE
  • Uniprot AC Q68FD5;
  • UniGene Mm.254588;
  • GeneID 67300
  • KEGG mmu:67300;
  • Mouse Clathrin heavy chain 1 Protein SEQUENCE
  • SEQUENCE 1675 AA; 191557 MW; 6C41EBD89EB7D56B CRC64;

    MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV

    VIIDMNDPSN PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK

    AHTMTDDVTF WKWISLNTVA LVTDNAVYHW SMEGESQPVK MFDRHSSLAG

    CQIINYRTDA KQKWLLLTGI SAQQNRVVGA MQLYSVDRKV SQPIEGHAAS

    FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN QPFPKKAVDV

    FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG

    ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR

    MAVRNNLAGA EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR

    FQSVPAQPGQ TSPLLQYFGI LLDQGQLNKY ESLELCRPVL QQGRKQLLEK

    WLKEDKLECS EELGDLVKSV DPTLALSVYL RANVPNKVIQ CFAETGQVQK

    IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE EPLADITQIV

    DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL

    GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW

    LVNYFGSLSV EDSLECLRAM LSANIRQNLQ ICVQVASKYH EQLSTQSLIE

    LFESFKSFEG LFYFLGSIVN FSQDPDVHFK YIQAACKTGQ IKEVERICRE

    SNCYDPERVK NFLKEAKLTD QLPLIIVCDR FDFVHDLVLY LYRNNLQKYI

    EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF STDELVAEVE

    KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY

    DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR

    KDPELWGSVL LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL

    PNELIELLEK IVLDNSVFSE HRNLQNLLIL TAIKADRTRV MEYINRLDNY

    DAPDIANIAI SNELFEEAFA IFRKFDVNTS AVQVLIEHIG NLDRAYEFAE

    RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV VQAANASGNW

    EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ

    VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST

    RTWKEVCFAC VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI

    TMLEAALGLE RAHMGMFTEL AILYSKFKPQ KMREHLELFW SRVNIPKVLR

    AAEQAHLWAE LVFLYDKYEE YDNAIITMMN HPTDAWKEGQ FKDIITKVAN

    VELYYKAIQF YLEFKPLLLN DLLMVLSPRL DHTRAVNYFS KVKQLPLVKP

    YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE

    KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA

    EELLQWFLQE EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI

    QVMKEYLTKV DKLDASESLR KEEEQATETQ PIVYGQPQLM LTAGPSVAVP

    PQAPFGYGYT APPYGQPQPG FGYSM

  • UCSC uc007kta.1; mouse.;



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