Human bMSH ELISA Kit

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  • Catalog
    E004841
  • species
    Human
  • GenebMSH
  • Standard CurveHuman bMSH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Beta-Melanocyte Stimulating Hormone (bMSH). No significant cross-reactivity or interference between Beta-Melanocyte Stimulating Hormone (bMSH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 15.3pg/mL.
  • Intended UseHuman bMSH ELISA Kit allows for the in vitro quantitative determination of bMSH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Melanocyte Stimulating Hormone (bMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Melanocyte Stimulating Hormone (bMSH) and unlabeled Beta-Melanocyte Stimulating Hormone (bMSH) (Standards or samples) with the pre-coated antibody specific to Beta-Melanocyte Stimulating Hormone (bMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Melanocyte Stimulating Hormone (bMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Melanocyte Stimulating Hormone (bMSH) in the sample.




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