Human bLPH ELISA Kit

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  • Alternative name

    BlpH ELISA KIT; BlpHblpH ELISA KIT;

  • Catalog
    E004837
  • species
    Human
  • GenebLPH
  • Standard CurveHuman bLPH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Beta-Lipotropic Hormone (bLPH). No significant cross-reactivity or interference between Beta-Lipotropic Hormone (bLPH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 2.48pg/mL.
  • Intended UseHuman bLPH ELISA Kit allows for the in vitro quantitative determination of bLPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Lipotropic Hormone (bLPH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Lipotropic Hormone (bLPH) and unlabeled Beta-Lipotropic Hormone (bLPH) (Standards or samples) with the pre-coated antibody specific to Beta-Lipotropic Hormone (bLPH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Lipotropic Hormone (bLPH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Lipotropic Hormone (bLPH) in the sample.




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