Mouse Clcn2 ELISA Kit

Alternative name
Mouse ClC-2 ELISA Kit;Mouse Clc2 ELISA Kit;Mouse AL118368 ELISA Kit;Mouse nmf240 ELISA Kit;Mouse chloride channel, voltage-sensitive 2 ELISA Kit;Mouse chloride channel protein 2 ELISA Kit;Mouse chloride channel 2 ELISA Kit;
Catalog
E048272
species
Mouse
GeneClcn2
Standard Curve
Other Species Human CLCN2 ELISA Kit Guinea Pig CLCN2 ELISA Kit Rabbit CLCN2 ELISA Kit Rat Clcn2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLCN2. No significant cross-reactivity or interference between CLCN2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLCN2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL
Intended UseMouse Clcn2 ELISA Kit allows for the in vitro quantitative determination of Clcn2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This CLCN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLCN2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CLCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLCN2 antibody and an CLCN2-HRP conjugate. The assay sample and buffer are incubated together with CLCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLCN2 concentration since CLCN2 from samples and CLCN2-HRP conjugate compete for the anti-CLCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLCN2 from the sample, fewer sites are left to bind CLCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLCN2 concentration in each sample is interpolated from this standard curve.
Mouse Chloride channel protein 2 Protein information
Uniprot ID CLCN2_MOUSE
Uniprot AC Q9R0A1; Q0VBC2; Q9WUJ9;
UniGene Mm.177761;
GeneID 12724
KEGG mmu:12724;
Mouse Chloride channel protein 2 Protein SEQUENCE
SEQUENCE 908 AA; 99447 MW; 6CF58033B60CA578 CRC64;
MAAATAAAAA AAAAGEGMEP RALQYEQTLM YGRYTQELGA FAKEEAARIR
LGGPEPWKGS PSARATPELL EYGQSRCARC RICSVRCHKF LVSRVGEDWI
FLVLLGLLMA LVSWAMDYAI AVCLQAQQWM SRGLNTNILL QYLAWVTYPV
VLITFSAGFT QILAPQAVGS GIPEMKTILR GVVLKEYLTL KTFVAKVIGL
TCALGSGMPL GKEGPFVHIA SMCAALLSKF LSLFGGIYEH ESRNTEMLAA
ACAVGVGCCF AAPIGGVLFS IEVTSTFFAV RNYWRGFFAA TFSAFIFRVL
AVWNRDEETI TALFKTRFRL DFPFDLQELP AFAVIGIASG FGGALFVYLN
RKIVQVMRKQ KTINRFLMRK RLLFPALVTL LISTLTFPPG FGQFMAGQLS
QKETLVTLFD NRTWVRQGLV EDLELPSTSQ AWSPPRANVF LTLVIFILMK
FWMSALATTI PVPCGAFMPV FVIGAAFGRL VGESMAAWFP DGIHTDSSTY
RIVPGGYAVV GAAALAGAVT HTVSTAVIVF ELTGQIAHIL PVMIAVILAN
AVAQSLQPSL YDSIIRIKKL PYLPELGWGR HQQYRVRVED IMVRDVPHVA
LSCTFRDLRL ALHRTKGRML ALVESPESMI LLGSIERSQV VALLGAQLSP
ARRRQHMQKL RKAQLSPPSD QESPPSSETS IRFQVNTEDS GFSGAHGQTH
KPLKPALKRG PSNSTSLQEG TTGNMESAGI ALRSLFCGSP PLEATSELEK
SESCDKRKLK RVRISLASDS DPEAEMSPEE ILEWEEQQLD EPVNFSDCKI
DPAPFQLVER TSLHKTHTIF SLLGVDHAYV TSIGRLIGIV TLKELRKAIE
GSVTAQGVKV RPPLASFRDS ATSSSDTETT EVHALWGPRS RHGLPREGTP
SDSDDKCQ
UCSC uc012ada.1; mouse.;

Category

Mouse Clcn2 ELISA Kit

Mouse ClC-2 ELISA Kit;Mouse Clc2 ELISA Kit;Mouse AL118368 ELISA Kit;Mouse nmf240 ELISA Kit;Mouse chloride channel, voltage-sensitive 2 ELISA Kit;Mouse chloride channel protein 2 ELISA Kit;Mouse chloride channel 2 ELISA Kit;