Mouse CCL-5 ELISA Kit

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  • Alternative name

    C-C motif chemokine 5 ELISA KIT; EoCP ELISA KIT; Eosinophil chemotactic cytokine ELISA KIT; SIS-delta ELISA KIT; Small-inducible cytokine A5 ELISA KIT; T cell-specific protein P228 ELISA KIT; TCP228 ELISA KIT; T-cell-specific protein RANTESCleaved into the following 2 chains:RANTES(3-68) ELISA KIT; RANTES(4-68)CCL5 ELISA KIT; D17S136E ELISA KIT; SCYA5 ELISA KIT; TCP228 ELISA KIT

  • Catalog
  • species
  • GeneCCL-5
  • Standard CurveMouse CCL-5 ELISA Kit
  • Other Species Human CCL 5 ELISA KitHuman CCL-5 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse CCL-5 ELISA Kit allows for the in vitro quantitative determination of CCL-5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This CCL5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CCL5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY CCL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL5 antibody and an CCL5-HRP conjugate. The assay sample and buffer are incubated together with CCL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL5 concentration since CCL5 from samples and CCL5-HRP conjugate compete for the anti-CCL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL5 from the sample, fewer sites are left to bind CCL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL5 concentration in each sample is interpolated from this standard curve.

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