Intended UseMouse Cxcl1 ELISA Kit allows for the in vitro quantitative determination of Cxcl1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalFor samples: For the quantitative determination of mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) concentrations in serum, plasma, tissue homogenates, cell culture supernates.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.