Mouse Prkg2 ELISA Kit

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  • Alternative name

    Mouse cGK 2 ELISA Kit;Mouse cGK2 ELISA Kit;Mouse cGMP-dependent protein kinase II ELISA Kit;Mouse cGKII ELISA Kit;Mouse Prkgr2 ELISA Kit;

  • Catalog
    E048045
  • species
    Mouse
  • GenePrkg2
  • Standard CurveMouse Prkg2 ELISA Kit
  • Other Species Human PRKG2 ELISA KitRat Prkg2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PRKG2. No significant cross-reactivity or interference between PRKG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRKG2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Prkg2 ELISA Kit allows for the in vitro quantitative determination of Prkg2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: PRKG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRKG2 antibody and an PRKG2-HRP conjugate. The assay sample and buffer are incubated together with PRKG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRKG2 concentration since PRKG2 from samples and PRKG2-HRP conjugate compete for the anti-PRKG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRKG2 from the sample, fewer sites are left to bind PRKG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRKG2 concentration in each sample is interpolated from this standard curve.
  • Mouse cGMP-dependent protein kinase 2 Protein information
  • Uniprot ID KGP2_MOUSE
  • Uniprot AC Q61410;
  • UniGene Mm.263002;
  • Mouse cGMP-dependent protein kinase 2 Protein SEQUENCE
  • SEQUENCE 762 AA; 87085 MW; 74F9D9A6835711A5 CRC64;

    MGNGSVKPKH AKHPDGHSGN LSNEALRSKV LELERELRRK DAELQEREYH

    LKELREQLAK QTVAIAELTE ELQSKCIQLN KLQDVIHVQG GSPLQASPDK

    VPLDVHRKTS GLVSLHSRRG AKAGVSAEPT TRTYDLNKPP EFSFEKARVR

    KDSSEKKLIT DALNKNQFLK RLDPQQIKDM VECMYGEKLS TGSYVIKQGE

    PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC TRTASVKAIT

    NVKTWALDRE VFQNIMRRTA QARDEEYRNF LRSVSLLKNL PEDKLTKIID

    CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ

    KGEYFGEKAL ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFDELQKY

    LEGYVATLNR DDEKRHAKRS MSSWKLSKAL SLEMIQLKEK VARFSSTSPF

    QNLEIIATLG VGGFGRVELV KVKNENVAFA MKCIRKKHIV DTKQQEHVYS

    EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS ILRDRGSFDE

    PTSKFCVACV TEAFDYLHLL GIIYRDLKPE NLILDADGYL KLVDFGFAKK

    IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF

    SGIDQMMTYN LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN

    GINDIKKHRW LNGFNWEGLK ARSLPSPLRR ELSGPIDHSY FDKYPPEKGV

    PPDEMSGWDK DF



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