Mouse Cfl2 ELISA Kit

Alternative name
Mouse cofilin, muscle isoform ELISA Kit;Mouse cofilin 2, muscle ELISA Kit;Mouse cofilin-2 ELISA Kit;
Catalog
E048041
species
Mouse
GeneCfl2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CFL2. No significant cross-reactivity or interference between Mouse CFL2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CFL2 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity15pg/mL
Intended UseMouse Cfl2 ELISA Kit allows for the in vitro quantitative determination of Cfl2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFL2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFL2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CFL2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CFL2. You can calculate the concentration of Mouse CFL2 in the samples by comparing the OD of the samples to the standard curve.
Mouse Cofilin-2 Protein information
Uniprot ID COF2_MOUSE
Uniprot AC P45591;
UniGene Mm.276826;
GeneID 12632
KEGG mmu:12632;
Mouse Cofilin-2 Protein SEQUENCE
SEQUENCE 166 AA; 18710 MW; 48B6D7E5AE9FE1CC CRC64;
MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE
EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV
FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG
EKLGGSVVVS LEGKPL
UCSC uc007nnx.1; mouse.;

Mouse Cfl2 ELISA Kit

Mouse cofilin, muscle isoform ELISA Kit;Mouse cofilin 2, muscle ELISA Kit;Mouse cofilin-2 ELISA Kit;

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