Mouse Cfl1 ELISA Kit

Alternative name
Mouse cofilin, non-muscle isoform ELISA Kit;Mouse AA959946 ELISA Kit;Mouse Cof ELISA Kit;Mouse cofilin 1, non-muscle ELISA Kit;Mouse cofilin-1 ELISA Kit;Mouse n-cofilin ELISA Kit;
Catalog
E048040
species
Mouse
GeneCfl1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CFL1. No significant cross-reactivity or interference between Mouse CFL1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CFL1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.188ng/mL
Intended UseMouse Cfl1 ELISA Kit allows for the in vitro quantitative determination of Cfl1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFL1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFL1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CFL1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CFL1. You can calculate the concentration of Mouse CFL1 in the samples by comparing the OD of the samples to the standard curve.
Mouse Cofilin-1 Protein information
Uniprot ID COF1_MOUSE
Uniprot AC P18760;
UniGene Mm.329655;
GeneID 12631
KEGG mmu:12631;
Mouse Cofilin-1 Protein SEQUENCE
SEQUENCE 166 AA; 18560 MW; 19834E8CA80747B2 CRC64;
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE
EGKEILVGDV GQTVDDPYTT FVKMLPDKDC RYALYDATYE TKESKKEDLV
FIFWAPENAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA
EKLGGSAVIS LEGKPL
UCSC uc008gdq.2; mouse.;

Mouse Cfl1 ELISA Kit

Mouse cofilin, non-muscle isoform ELISA Kit;Mouse AA959946 ELISA Kit;Mouse Cof ELISA Kit;Mouse cofilin 1, non-muscle ELISA Kit;Mouse cofilin-1 ELISA Kit;Mouse n-cofilin ELISA Kit;

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