Mouse Cfb ELISA Kit

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  • Alternative name

    Mouse C3/C5 convertase ELISA Kit;Mouse Bf ELISA Kit;Mouse H2-Bf ELISA Kit;

  • Catalog
    E048037
  • species
    Mouse
  • GeneCfb
  • Other Species Human CFB ELISA KitMouse pre-CFB ELISA KitBovine CFB ELISA KitPorcine CFB ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Mouse CFB. No significant cross-reactivity or interference between Mouse CFB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.938ng/mL
  • Intended UseMouse Cfb ELISA Kit allows for the in vitro quantitative determination of Cfb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CFB concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CFB. You can calculate the concentration of CFB in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Complement factor B Bb fragment Protein information
  • Uniprot ID CFAB_MOUSE
  • Uniprot AC P04186;
  • UniGene Mm.653;
  • Mouse Complement factor B Bb fragment Protein SEQUENCE
  • SEQUENCE 761 AA; 85004 MW; 9E9015448A61020E CRC64;

    MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ

    GGQALEYLCP SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC

    PRPQDFENGE FWPRSPFYNL SDQISFQCYD GYVLRGSANR TCQENGRWDG

    QTAICDDGAG YCPNPGIPIG TRKVGSQYRL EDIVTYHCSR GLVLRGSQKR

    KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE GADAEDGHSP

    GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY

    GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT

    NTKRALQAVY SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ

    DIRALLDIGR DPKNPREDYL DVYVFGVGPL VDSVNINALA SKKDNEHHVF

    KVKDMEDLEN VFYQMIDETK SLSLCGMVWE HKKGNDYHKQ PWQAKISVTR

    PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG QRRDLEIEEV

    LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT

    TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK

    ASCERDATKA QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL

    IVHKRSRFIQ VGVISWGVVD VCRDQRRQQL VPSYARDFHI NLFQVLPWLK

    DKLKDEDLGF L




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