Mouse Cerk ELISA Kit

Alternative name
Mouse mCERK ELISA Kit;Mouse acylsphingosine kinase ELISA Kit;Mouse AI848610 ELISA Kit;Mouse D330016D08Rik ELISA Kit;Mouse mKIAA1646 ELISA Kit;Mouse ceramide kinase ELISA Kit;
Catalog
E048005
species
Mouse
GeneCerk
Standard Curve
Other Species Human CERK ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CERK. No significant cross-reactivity or interference between CERK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CERK and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Cerk ELISA Kit allows for the in vitro quantitative determination of Cerk , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CERK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CERK antibody and an CERK-HRP conjugate. The assay sample and buffer are incubated together with CERK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CERK concentration since CERK from samples and CERK-HRP conjugate compete for the anti-CERK antibody binding site. Since the number of sites is limited, as more sites are occupied by CERK from the sample, fewer sites are left to bind CERK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CERK concentration in each sample is interpolated from this standard curve.
Mouse Ceramide kinase Protein information
Uniprot ID CERK1_MOUSE
Uniprot AC Q8K4Q7; Q52KP2;
UniGene Mm.222685;
GeneID 223753
KEGG mmu:223753;
Mouse Ceramide kinase Protein SEQUENCE
SEQUENCE 531 AA; 59843 MW; C5A030D422E3AA10 CRC64;
MGAMGAAEPL HSVLWVKRRR CAVSLEPARA LLRWWRSPEP GPSAPGADAR
SVLVSEIIAV EEKDDCEKHA SSGRWHKMEN PFAFTVHRVK RVRHHRWKWA
RVTFWSADEQ LCHLWLQTLR GLLESLTSRP KHLLVFINPF GGKGQGKRIY
EKTVAPLFTL ASITTEIIIT EHANQAKETL YEINTDSYDG IVCVGGDGMF
SEVLHGVIGR TQQSAGIDPN HPRAVLVPST LRIGIIPAGS TDCVCYSTVG
TNDAETSALH IIIGDSLAID VSSVHYHNTL LRYSVSLLGY GFYGDLIKDS
EKKRWMGLVR YDFSGLKTFL SHQYYEGTLS FLPAQHTVGS PRDNKPCRAG
CFVCRQSKQQ LEEEEKKALY GLENAEEMEE WQVTCGKFLA INATNMSCAC
PRSPGGLSPF AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QEDQFDFTFV
EVYRVKKFHF TSKHVEDEDN DSKEQEKQKF GKICKDRPSC TCSASRSSWN
CDGEVMHSPA IEVRVHCQLV RLFARGIEEE S
UCSC uc007xdx.2; mouse.;

Category

Mouse Cerk ELISA Kit

Mouse mCERK ELISA Kit;Mouse acylsphingosine kinase ELISA Kit;Mouse AI848610 ELISA Kit;Mouse D330016D08Rik ELISA Kit;Mouse mKIAA1646 ELISA Kit;Mouse ceramide kinase ELISA Kit;