Mouse Cenpb ELISA Kit

Alternative name
Mouse centromere protein B ELISA Kit;Mouse CENP-B ELISA Kit;Mouse major centromere autoantigen B ELISA Kit;Mouse centromere autoantigen B ELISA Kit;
Catalog
E047928
species
Mouse
GeneCenpb
Other Species Human CENPB ELISA Kit Hamster CENPB ELISA Kit Sheep CENPB ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CENPB. No significant cross-reactivity or interference between Mouse CENPB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CENPB and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Cenpb ELISA Kit allows for the in vitro quantitative determination of Cenpb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CENPB in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CENPB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CENPB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CENPB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CENPB. You can calculate the concentration of Mouse CENPB in the samples by comparing the OD of the samples to the standard curve.
Mouse Major centromere autoantigen B Protein information
Uniprot ID CENPB_MOUSE
Uniprot AC P27790; Q7TSG8;
UniGene Mm.440169;
GeneID 12616
KEGG mmu:12616;
Mouse Major centromere autoantigen B Protein SEQUENCE
SEQUENCE 599 AA; 65381 MW; EBDB7C76BA87DC73 CRC64;
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR
AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL
KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVACSGVTR SRARSSAPRA
PAAPAGPATV PSEGSGGSTP GWHTREEQPP SVAEGYASQD VFSATETSLW
YDFLSDQASG LWGGDGPARQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP
RAGQGGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA
AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE
GQDPSGLQLG LVEALHFVAA AWQAVEPSDI ATCFREAGFG GGLNATITTS
FKSEGEEEEE EEEEEEEEEE EEGEGEEEEE EEEEGEEEGG EGEEEGEEEV
EEEGEVDDSD EEEEESSSEG LEAEDWAQGV VEASGGFGGY SVQEEAQFPT
LHFLEGGEDS DSDSDEEEDD EEEDEEDEDE EDDEDGDEVP VPSFGEAMAY
FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS
UCSC uc008mkx.1; mouse.;

Category

Mouse Cenpb ELISA Kit

Mouse centromere protein B ELISA Kit;Mouse CENP-B ELISA Kit;Mouse major centromere autoantigen B ELISA Kit;Mouse centromere autoantigen B ELISA Kit;