Mouse Cdc123 ELISA Kit

Alternative name
Mouse AA959893 ELISA Kit;Mouse G431001I09Rik ELISA Kit;Mouse cell division cycle 123 ELISA Kit;Mouse cell division cycle protein 123 homolog ELISA Kit;Mouse cell division cycle 123 homolog ELISA Kit;
Catalog
E047890
species
Mouse
GeneCdc123
Standard Curve
Other Species Bovine CDC123 ELISA Kit Human CDC123 ELISA Kit Monkey CDC123 ELISA Kit Rat Cdc123 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CDC123. No significant cross-reactivity or interference between CDC123and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC123 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cdc123 ELISA Kit allows for the in vitro quantitative determination of Cdc123 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses||This CDC123 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CDC123. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CDC123 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC123 antibody and an CDC123-HRP conjugate. The assay sample and buffer are incubated together with CDC123-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theCDC123 concentration since CDC123 from samples and CDC123-HRP conjugate compete for the anti-CDC123 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC123 from the sample, fewer sites are left to bind CDC123-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC123 concentration in each sample is interpolated from this standard curve.
Mouse Cell division cycle protein 123 homolog Protein information
Uniprot ID CD123_MOUSE
Uniprot AC Q8CII2; Q3TTR0; Q8VE51;
UniGene Mm.181490;
GeneID 98828
KEGG mmu:98828;
Mouse Cell division cycle protein 123 homolog Protein SEQUENCE
SEQUENCE 336 AA; 38816 MW; 1E59461A424B7A76 CRC64;
MKKEHVSHCQ FSAWYPLFRS LTIKSVILPL PQNVKDYLLD DGTLVVSGRE
DPPTCSQSDS GNEAEETQWS DDESTATLTA PEFPEFNTQV QEAINSLGGS
VFPKLNWSAP RDAYWIAMNS SLKCKTLSDI FLLFKSSDFI THDFTQPFIH
CTDDSPDPCI EYELVLRKWC ELIPGAEFRC FVKENKLIGI SQRDYTQYYD
HISKQKEEIC RCIQDFFKEH LQYKFLDEDF VFDIYRDSRG KVWLIDFNPF
GEVTDSLLFT WEELTSENNL RGEVTEGDAQ EQDSPAFRCT NSEVTVQPSP
YLSFGLPKDF VDLSTGEDAH KLIDFLKLKR NEQEDD
UCSC uc008ifu.1; mouse. [Q8CII2-2];

Category

Mouse Cdc123 ELISA Kit

Mouse AA959893 ELISA Kit;Mouse G431001I09Rik ELISA Kit;Mouse cell division cycle 123 ELISA Kit;Mouse cell division cycle protein 123 homolog ELISA Kit;Mouse cell division cycle 123 homolog ELISA Kit;