-
Alternative name
Human beta-defensin 27 ELISA Kit;Human DEFB-27 ELISA Kit;Human Defensin, beta 127 ELISA Kit;Human C20orf73 ELISA Kit;Human DEFB27 ELISA Kit;Human DEF-27 ELISA Kit;Human bA530N10.2 ELISA Kit;Human hBD-27 ELISA Kit;Human defensin beta 127 ELISA Kit;Human beta-defensin 127 ELISA Kit;
-
Catalog
E004784
-
species
Human
-
GeneDEFB127
-
Other Species
Mouse DEFb127 ELISA Kit
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity<56.25pg/ml
-
Intended UseHuman DEFB127 ELISA Kit allows for the in vitro quantitative determination of DEFB127 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human DEFB127 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DEFB127 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DEFB127 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DEFB127 can be calculated.
-
Human Beta-defensin 127 Protein information
-
Uniprot ID
DB127_HUMAN
-
Uniprot AC
Q9H1M4; Q14DW7;
-
UniGene
Hs.99362;
-
GeneID
140850
-
KEGG
hsa:140850;
-
Human Beta-defensin 127 Protein SEQUENCE
-
SEQUENCE 99 AA; 11343 MW; C9CBDB27D17AF380 CRC64;
MGLFMIIAIL LFQKPTVTEQ LKKCWNNYVQ GHCRKICRVN EVPEALCENG
RYCCLNIKEL
EACKKITKPP RPKPATLALT LQDYVTIIEN FPSLKTQST
-
UCSC
uc002wcy.2; human.;
-