Mouse Cables2 ELISA Kit

Alternative name
Mouse interactor with CDK3 2 ELISA Kit;Mouse ik3-2 ELISA Kit;Mouse CDK5 and Abl enzyme substrate 2 ELISA Kit;Mouse CDK5 and ABL1 enzyme substrate 2 ELISA Kit;
Catalog
E047819
species
Mouse
GeneCables2
Standard Curve
Other Species Human CABLES2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CABLES2. No significant cross-reactivity or interference between CABLES2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CABLES2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cables2 ELISA Kit allows for the in vitro quantitative determination of Cables2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CABLES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CABLES2 antibody and an CABLES2-HRP conjugate. The assay sample and buffer are incubated together with CABLES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CABLES2 concentration since CABLES2 from samples and CABLES2-HRP conjugate compete for the anti-CABLES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CABLES2 from the sample, fewer sites are left to bind CABLES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CABLES2 concentration in each sample is interpolated from this standard curve.
Mouse CDK5 and ABL1 enzyme substrate 2 Protein information
Uniprot ID CABL2_MOUSE
Uniprot AC Q8K3M5; A2ABW9;
UniGene Mm.386884;
GeneID 252966
KEGG mmu:252966;
Mouse CDK5 and ABL1 enzyme substrate 2 Protein SEQUENCE
SEQUENCE 476 AA; 52082 MW; 9557AEFE00B668EC CRC64;
MAAAAAGGAP GPAPGPSRPA PAARNPPAVP RRRGDSRRRQ AALFFLNNIS
LDGRPPSLGP GGEKPAPPPP PPTEAREAPA PPPAPPGGLP GLPARPAPQG
LLSPTTAPAG LGLDGQRQRR RVTSQRCSLE FLEDTVGCAS VQRTKHASGS
PRHKGLKKTH FIKNMRQYDT KNSRIVLICA KRSLCAAFSV LPYGEGLRIS
DLRVDSQKQR HPSGGVSVSS EMVFELEGVE LGADGKVVSY AKFLYPTNAL
VIHKNDSHGL LPQPRPSIPR APPGSRHKPV PTKSTPAGTE LGSDGGDAVE
YNPNLLDDPQ WPCGKHKRVL IFASYMTTVI EYVKPADLKK DMNETFREKF
PHIKLTLSKI RSLKREMRNL SEECSLEPVT VSMAYVYFEK LVLQGKLNKQ
NRKLCAGACV LLAAKISSDL RKSEVKQLID KLEERFRFNR KDLIGFEFTV
LVALELALYL PENQVLPHYR RLTQQF
UCSC uc008oit.1; mouse.;

Category

Mouse Cables2 ELISA Kit

Mouse interactor with CDK3 2 ELISA Kit;Mouse ik3-2 ELISA Kit;Mouse CDK5 and Abl enzyme substrate 2 ELISA Kit;Mouse CDK5 and ABL1 enzyme substrate 2 ELISA Kit;