Mouse Cd81 ELISA Kit

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  • Alternative name

    Mouse 26 kDa cell surface protein TAPA-1 ELISA Kit;Mouse target of the antiproliferative antibody 1 ELISA Kit;Mouse Tapa1 ELISA Kit;Mouse Tapa-1 ELISA Kit;Mouse Tspan28 ELISA Kit;Mouse CD81 antigen ELISA Kit;

  • Catalog
    E047772
  • species
    Mouse
  • GeneCd81
  • Standard CurveMouse Cd81 ELISA Kit
  • Other Species Human CD81 ELISA KitBovine CD81 ELISA KitRat Cd81 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD81. No significant cross-reactivity or interference between CD81 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD81 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Cd81 ELISA Kit allows for the in vitro quantitative determination of Cd81 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Intended Uses: This CD81 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD81. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CD81 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD81 antibody and an CD81-HRP conjugate. The assay sample and buffer are incubated together with CD81-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD81 concentration since CD81 from samples and CD81-HRP conjugate compete for the anti-CD81 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD81 from the sample, fewer sites are left to bind CD81-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD81 concentration in each sample is interpolated from this standard curve.
  • Mouse CD81 antigen Protein information
  • Uniprot ID CD81_MOUSE
  • Uniprot AC P35762; Q91V78;
  • UniGene Mm.806;
  • GeneID 12520
  • KEGG mmu:12520;
  • Mouse CD81 antigen Protein SEQUENCE
  • SEQUENCE 236 AA; 25815 MW; 7BDCB792E7F9FA4E CRC64;

    MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTSLLYLELG

    NKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI

    LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVMDDDA NNAKAVVKTF

    HETLNCCGSN ALTTLTTTIL RNSLCPSGGN ILTPLLQQDC HQKIDELFSG

    KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY

  • UCSC uc009kou.2; mouse.;


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