Mouse Cd48 ELISA Kit

Alternative name
Mouse BCM1 surface antigen ELISA Kit;Mouse BLAST-1 ELISA Kit;Mouse HM48-1 ELISA Kit;Mouse MRC OX-45 surface antigen ELISA Kit;Mouse SLAM family member 2 ELISA Kit;Mouse SLAMF2 ELISA Kit;Mouse signaling lymphocytic activation molecule 2 ELISA Kit;Mouse Sgp-60 ELISA Kit;Mouse Bcm-1 ELISA Kit;Mouse AI449234 ELISA Kit;Mouse AW610730 ELISA Kit;Mouse BCM1 ELISA Kit;Mouse BLAST ELISA Kit;Mouse BLAST1 ELISA Kit;Mouse MEM-102 ELISA Kit;Mouse CD48 antigen ELISA Kit;
Catalog
E047726
species
Mouse
GeneCd48
SpecificityNatural and recombinant mouse CD48
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Cd48 ELISA Kit allows for the in vitro quantitative determination of Cd48 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD48 has been precoated onto 96-well plates. Standards(NSO, F23-S217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD48 amount of sample captured in plate. Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.
Mouse CD48 antigen Protein information
Uniprot ID CD48_MOUSE
Uniprot AC P18181; Q545K2;
UniGene Mm.1738;
GeneID 12506
KEGG mmu:12506;
Mouse CD48 antigen Protein SEQUENCE
SEQUENCE 240 AA; 27383 MW; F3BF6987A9E9C71E CRC64;
MCFIKQGWCL VLELLLLPLG TGFQGHSIPD INATTGSNVT LKIHKDPLGP
YKRITWLHTK NQKILEYNYN STKTIFESEF KGRVYLEENN GALHISNVRK
EDKGTYYMRV LRETENELKI TLEVFDPVPK PSIEINKTEA STDSCHLRLS
CEVKDQHVDY TWYESSGPFP KKSPGYVLDL IVTPQNKSTF YTCQVSNPVS
SKNDTVYFTL PCDLARSSGV CWTATWLVVT TLIIHRILLT
UCSC uc007doz.1; mouse.;

Mouse Cd48 ELISA Kit

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