Mouse Cd33 ELISA Kit

Alternative name
Mouse sialic acid-binding Ig-like lectin 3 ELISA Kit;Mouse Siglec-3 ELISA Kit;Mouse Siglec3 ELISA Kit;Mouse gp67 ELISA Kit;Mouse CD33 antigen ELISA Kit;Mouse myeloid cell surface antigen CD33 ELISA Kit;
Catalog
E047693
species
Mouse
GeneCd33
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CD33. No significant cross-reactivity or interference between Mouse CD33 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Cd33 ELISA Kit allows for the in vitro quantitative determination of Cd33 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD33. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CD33 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CD33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CD33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Myeloid cell surface antigen CD33 Protein information
Uniprot ID CD33_MOUSE
Uniprot AC Q63994; A2RT59; Q63997;
UniGene Mm.140157;
GeneID 12489
KEGG mmu:12489;
Mouse Myeloid cell surface antigen CD33 Protein SEQUENCE
SEQUENCE 403 AA; 44824 MW; F1FE6D5C393F0FF1 CRC64;
MLWPLPLFLL CAGSLAQDLE FQLVAPESVT VEEGLCVHVP CSVFYPSIKL
TLGPVTGSWL RKGVSLHEDS PVATSDPRQL VQKATQGRFQ LLGDPQKHDC
SLFIRDAQKN DTGMYFFRVV REPFVRYSYK KSQLSLHVTS LSRTPDIIIP
GTLEAGYPSN LTCSVPWACE QGTPPTFSWM STALTSLSSR TTDSSVLTFT
PQPQDHGTKL TCLVTFSGAG VTVERTIQLN VTRKSGQMRE LVLVAVGEAT
VKLLILGLCL VFLIVMFCRR KTTKLSVHMG CENPIKRQEA ITSYNHCLSP
TASDAVTPGC SIHRLISRTP RCTAILRIQD PYRRTHLRNR AVSTLRFPWI
SWEGSLRSTQ RSKCTKLCSP VKNLCPLWLP VDNSCIPLIP EWVMLLCVSL
TLS
UCSC uc009gna.1; mouse. [Q63994-1];

Category

Mouse Cd33 ELISA Kit

Mouse sialic acid-binding Ig-like lectin 3 ELISA Kit;Mouse Siglec-3 ELISA Kit;Mouse Siglec3 ELISA Kit;Mouse gp67 ELISA Kit;Mouse CD33 antigen ELISA Kit;Mouse myeloid cell surface antigen CD33 ELISA Kit;