Mouse Nrp1 ELISA Kit

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  • Alternative name

    Mouse A5 protein ELISA Kit;Mouse Nrp ELISA Kit;Mouse C530029I03 ELISA Kit;Mouse NP-1 ELISA Kit;Mouse NPN-1 ELISA Kit;Mouse Npn1 ELISA Kit;Mouse neuropilin 1 ELISA Kit;Mouse neuropilin-1 ELISA Kit;Mouse Neuropilin-1 precursor (A5 protein) ELISA Kit;Mouse transmembrane receptor ELISA Kit;

  • Catalog
    E047683
  • species
    Mouse
  • GeneNrp1
  • Other Species Human NRP1 ELISA KitChicken NRP1 ELISA KitRat Nrp1 ELISA Kit
  • SpecificityThe Mouse CD304 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD304 proteins within the range of 780 pg/ml - 50000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity20 pg/ml.
  • Intended UseMouse Nrp1 ELISA Kit allows for the in vitro quantitative determination of Nrp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Mouse CD304 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD304 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD304 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD304 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD304 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD304 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
  • Mouse Neuropilin-1 Protein information
  • Uniprot ID NRP1_MOUSE
  • Uniprot AC P97333; Q6PAR3;
  • UniGene Mm.271745;
  • GeneID 18186
  • KEGG mmu:18186;
  • Mouse Neuropilin-1 Protein SEQUENCE
  • SEQUENCE 923 AA; 103000 MW; 23209818C5C42137 CRC64;

    MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP

    SEKCEWLIQA PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW

    GKFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN

    YTAPTGVIKS PGFPEKYPNS LECTYIIFAP KMSEIILEFE SFDLEQDSNP

    PGGMFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS GVLSMVFYTD

    SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN

    WSVERSRLNY PENGWTPGED SYKEWIQVDL GLLRFVTAVG TQGAISKETK

    KKYYVKTYRV DISSNGEDWI SLKEGNKAII FQGNTNPTDV VLGVFSKPLI

    TRFVRIKPVS WETGISMRFE VYGCKITDYP CSGMLGMVSG LISDSQITAS

    NQADRNWMPE NIRLVTSRTG WALPPSPHPY TNEWLQVDLG DEKIVRGVII

    QGGKHRENKV FMRKFKIAYS NNGSDWKTIM DDSKRKAKSF EGNNNYDTPE

    LRTFSPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEAPTA GPTTPNGNPV

    DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC

    EFGWGSHKTF CHWEHDSHAQ LRWSVLTSKT GPIQDHTGDG NFIYSQADEN

    QKGKVARLVS PVVYSQSSAH CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ

    LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI FEGEIGKGNL GGIAVDDISI

    NNHISQEDCA KPTDLDKKNT EIKIDETGST PGYEGEGEGD KNISRKPGNV

    LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN

    FELVDGVKLK KDKLNPQSNY SEA

  • UCSC uc009nzr.2; mouse.;


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