Mouse Cd274 ELISA Kit

Alternative name
Mouse PD-L1 ELISA Kit;Mouse PDCD1 ligand 1 ELISA Kit;Mouse Programmed death ligand 1 ELISA Kit;Mouse B7 homolog 1 ELISA Kit;Mouse B7-H1 ELISA Kit;Mouse B7h1 ELISA Kit;Mouse Pdcd1l1 ELISA Kit;Mouse Pdcd1lg1 ELISA Kit;Mouse Pdl1 ELISA Kit;Mouse A530045L16Rik ELISA Kit;Mouse CD274 antigen ELISA Kit;Mouse programmed cell death 1 ligand 1 ELISA Kit;
Catalog
E047669
species
Mouse
GeneCd274
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse CD274. No significant cross-reactivity or interference between mouse CD274 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse CD274 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseMouse Cd274 ELISA Kit allows for the in vitro quantitative determination of Cd274 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD274 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD274 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD274 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD274 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Programmed cell death 1 ligand 1 Protein information
Uniprot ID PD1L1_MOUSE
Uniprot AC Q9EP73;
UniGene Mm.245363;
GeneID 60533
KEGG mmu:60533;
Mouse Programmed cell death 1 ligand 1 Protein SEQUENCE
SEQUENCE 290 AA; 32780 MW; AB7C46CF853EBB02 CRC64;
MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL
LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ
ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH
ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA
TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL
IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET
UCSC uc008hdi.2; mouse.;

Mouse Cd274 ELISA Kit

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