Mouse Cd200 ELISA Kit

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  • Alternative name

    Mouse MRC OX-2 antigen ELISA Kit;Mouse Mox2 ELISA Kit;

  • Catalog
    E047632
  • species
    Mouse
  • GeneCd200
  • Other Species Human CD200 ELISA KitRat Cd200 ELISA Kit
  • SpecificityNatural and recombinant mouse CD200
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10 pg/ml
  • Intended UseMouse Cd200 ELISA Kit allows for the in vitro quantitative determination of Cd200 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: mouse CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD200 has been precoated onto 96-well plates. Standards(NSO, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD200 amount of sample captured in plate. Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
  • Mouse OX-2 membrane glycoprotein Protein information
  • Uniprot ID OX2G_MOUSE
  • Uniprot AC O54901; O54816; Q9JHD5;
  • UniGene Mm.245851;
  • Mouse OX-2 membrane glycoprotein Protein SEQUENCE
  • SEQUENCE 278 AA; 31256 MW; 0D06A2DE0C60DF5A CRC64;

    MGSLVFRRPF CHLSTYSLIW GMAAVALSTA QVEVVTQDER KALHTTASLR

    CSLKTSQEPL IVTWQKKKAV SPENMVTYSK THGVVIQPAY KDRINVTELG

    LWNSSITFWN TTLEDEGCYM CLFNTFGSQK VSGTACLTLY VQPIVHLHYN

    YFEDHLNITC SATARPAPAI SWKGTGTGIE NSTESHFHSN GTTSVTSILR

    VKDPKTQVGK EVICQVLYLG NVIDYKQSLD KGFWFSVPLL LSIVSLVILL

    VLISILLYWK RHRNQERGES SQGMQRMK





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