Mouse Siglec1 ELISA Kit

Alternative name
Mouse sheep erythrocyte receptor ELISA Kit;Mouse SER ELISA Kit;Mouse sialic acid-binding Ig-like lectin 1 ELISA Kit;Mouse Siglec-1 ELISA Kit;Mouse Sa ELISA Kit;Mouse Sn ELISA Kit;Mouse Cd169 ELISA Kit;Mouse sialic acid binding Ig-like lectin 1, sialoadhesin ELISA Kit;Mouse sialoadhesin ELISA Kit;
Catalog
E047620
species
Mouse
GeneSiglec1
Other Species Human SIGLEC1 ELISA Kit Porcine SIGLEC1 ELISA Kit
SpecificityThe Mouse CD169 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD169 proteins within the range of 156 pg/ml - 10000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity10 pg/ml.
Intended UseMouse Siglec1 ELISA Kit allows for the in vitro quantitative determination of Siglec1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Mouse CD169 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD169 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD169 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD169 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD169 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD169 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Mouse Sialoadhesin Protein information
Uniprot ID SN_MOUSE
Uniprot AC Q62230; D3YVZ3; D3YVZ4; O55216; Q62228; Q62229;
UniGene Mm.1374;
GeneID 20612
KEGG mmu:20612;
Mouse Sialoadhesin Protein SEQUENCE
SEQUENCE 1695 AA; 182979 MW; AF704F68C40E113A CRC64;
MCVLFSLLLL ASVFSLGQTT WGVSSPKNVQ GLSGSCLLIP CIFSYPADVP
VSNGITAIWY YDYSGKRQVV IHSGDPKLVD KRFRGRAELM GNMDHKVCNL
LLKDLKPEDS GTYNFRFEIS DSNRWLDVKG TTVTVTTDPS PPTITIPEEL
REGMERNFNC STPYLCLQEK QVSLQWRGQD PTHSVTSSFQ SLEPTGVYHQ
TTLHMALSWQ DHGRTLLCQF SLGAHSSRKE VYLQVPHAPK GVEILLSSSG
RNILPGDPVT LTCRVNSSYP AVSAVQWARD GVNLGVTGHV LRLFSAAWND
SGAYTCQATN DMGSLVSSPL SLHVFMAEVK MNPAGPVLEN ETVTLLCSTP
KEAPQELRYS WYKNHILLED AHASTLHLPA VTRADTGFYF CEVQNAQGSE
RSSPLSVVVR YPPLTPDLTT FLETQAGLVG ILHCSVVSEP LATVVLSHGG
LTLASNSGEN DFNPRFRISS APNSLRLEIR DLQPADSGEY TCLAVNSLGN
STSSLDFYAN VARLLINPSA EVVEGQAVTL SCRSGLSPAP DTRFSWYLNG
ALLLEGSSSS LLLPAASSTD AGSYYCRTQA GPNTSGPSLP TVLTVFYPPR
KPTFTARLDL DTSGVGDGRR GILLCHVDSD PPAQLRLLHK GHVVATSLPS
RCGSCSQRTK VSRTSNSLHV EIQKPVLEDE GVYLCEASNT LGNSSAAASF
NAKATVLVIT PSNTLREGTE ANLTCNVNQE VAVSPANFSW FRNGVLWTQG
SLETVRLQPV ARTDAAVYAC RLLTEDGAQL SAPVVLSVLY APDPPKLSAL
LDVGQGHMAV FICTVDSYPL AHLSLFRGDH LLATNLEPQR PSHGRIQAKA
TANSLQLEVR ELGLVDSGNY HCEATNILGS ANSSLFFQVR GAWVQVSPSP
ELREGQAVVL SCQVPTGVSE GTSYSWYQDG RPLQESTSST LRIAAISLRQ
AGAYHCQAQA PDTAIASLAA PVSLHVSYTP RHVTLSALLS TDPERLGHLV
CSVQSDPPAQ LQLFHRNRLV ASTLQGADEL AGSNPRLHVT VLPNELRLQI
HFPELEDDGT YTCEASNTLG QASAAADFDA QAVRVTVWPN ATVQEGQQVN
LTCLVWSTHQ DSLSYTWYKG GQQLLGARSI TLPSVKVLDA TSYRCGVGLP
GHAPHLSRPV TLDVLHAPRN LRLTYLLETQ GRQLALVLCT VDSRPPAQLT
LSHGDQLVAS STEASVPNTL RLELQDPRPS NEGLYSCSAH SPLGKANTSL
ELLLEGVRVK MNPSGSVPEG EPVTVTCEDP AALSSALYAW FHNGHWLQEG
PASSLQFLVT TRAHAGAYFC QVHDTQGTRS SRPASLQILY APRDAVLSSF
RDSRTRLMVV IQCTVDSEPP AEMVLSHNGK VLAASHERHS SASGIGHIQV
ARNALRLQVQ DVTLGDGNTY VCTAQNTLGS ISTTQRLLTE TDIRVTAEPG
LDVPEGTALN LSCLLPGGSG PTGNSSFTWF WNRHRLHSAP VPTLSFTPVV
RAQAGLYHCR ADLPTGATTS APVMLRVLYP PKTPTLIVFV EPQGGHQGIL
DCRVDSEPLA ILTLHRGSQL VASNQLHDAP TKPHIRVTAP PNALRVDIEE
LGPSNQGEYV CTASNTLGSA SASAYFGTRA LHQLQLFQRL LWVLGFLAGF
LCLLLGLVAY HTWRKKSSTK LNEDENSAEM ATKKNTIQEE VVAAL
UCSC uc008mkq.1; mouse. [Q62230-2];

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Mouse Siglec1 ELISA Kit

Mouse sheep erythrocyte receptor ELISA Kit;Mouse SER ELISA Kit;Mouse sialic acid-binding Ig-like lectin 1 ELISA Kit;Mouse Siglec-1 ELISA Kit;Mouse Sa ELISA Kit;Mouse Sn ELISA Kit;Mouse Cd169 ELISA Kit;Mouse sialic acid binding Ig-like lectin 1, sialoadhesin ELISA Kit;Mouse sialoadhesin ELISA Kit;