Mouse Cd40lg ELISA Kit

Alternative name
Mouse CD40-L ELISA Kit;Mouse T-cell antigen Gp39 ELISA Kit;Mouse TNF-related activation protein ELISA Kit;Mouse TRAP ELISA Kit;Mouse tumor necrosis factor ligand superfamily member 5 ELISA Kit;Mouse Cd40l ELISA Kit;Mouse Tnfsf5 ELISA Kit;Mouse CD154 ELISA Kit;Mouse HIGM1 ELISA Kit;Mouse IGM ELISA Kit;Mouse IMD3 ELISA Kit;Mouse Ly-62 ELISA Kit;Mouse Ly62 ELISA Kit;Mouse T-BAM ELISA Kit;Mouse Tnlg8b ELISA Kit;Mouse gp39 ELISA Kit;Mouse CD40 ligand ELISA Kit;Mouse tumor necrosis factor ligand 8b ELISA Kit;
Catalog
E047607
species
Mouse
GeneCd40lg
SpecificityThe Mouse CD154 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD154 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity10 pg/ml.
Intended UseMouse Cd40lg ELISA Kit allows for the in vitro quantitative determination of Cd40lg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Mouse CD154 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD154 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD154 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD154 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD154 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD154 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Mouse CD40 ligand, soluble form Protein information
Uniprot ID CD40L_MOUSE
Uniprot AC P27548;
UniGene Mm.4861;
GeneID 21947
KEGG mmu:21947;
Mouse CD40 ligand, soluble form Protein SEQUENCE
SEQUENCE 260 AA; 29370 MW; 7E1AC117473672AD CRC64;
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL
DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL
NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN
LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS
ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV
GFSSFGLLKL
UCSC uc009thb.1; mouse.;

Mouse Cd40lg ELISA Kit

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