Human Beta-APP42 ELISA Kit

Contact Us
  • Catalog
    E004757
  • species
    Human
  • GeneBeta-APP42
  • Standard CurveHuman Beta-APP42 ELISA Kit
  • Other Species Mouse Beta-APP42 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural human Beta-amyloid protein 42. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman Beta-APP42 ELISA Kit allows for the in vitro quantitative determination of Beta-APP42 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-amyloid protein 42. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beta-amyloid protein 42 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beta-amyloid protein 42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Beta-amyloid protein 42 in the samples is then determined by comparing the O.D. of the samples to the standard curve.




tag

Inquiry About Human Beta-APP42 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!