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Alternative name
Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APPI ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid protein ELISA KIT; Cerebral vascular amyloid peptide ELISA KIT; CVAP ELISA KIT; PreA4 ELISA KIT; Protease nexin-II ELISA KIT; PN-IIAPP ELISA KIT; A4 ELISA KIT; AD1 ELISA KIT; APP ELISA KIT; CVAP ELISA KIT; PN-II ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT
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Catalog
E004755
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species
Human
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Genebeta-APP
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Standard Curve
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Other Species
Human beta APP ELISA KitMouse beta-APP ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human beta-APP. No significant cross-reactivity or interference between human beta-APP and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human beta-APP is typically less than 11.75 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined th
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Intended UseHuman beta-APP ELISA Kit allows for the in vitro quantitative determination of beta-APP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-APP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-APP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-APP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-APP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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