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Alternative name
C-C motif chemokine 21a ELISA KIT; 6Ckine ELISA KIT; Beta-chemokine exodus-2 ELISA KIT; Small-inducible cytokine A21a ELISA KIT; Thymus-derived chemotactic agent 4 ELISA KIT; TCA4Ccl21a ELISA KIT; Scya21 ELISA KIT; Scya21a ELISA KIT; TCA4 ELISA KIT
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Catalog
E047547
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species
Mouse
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GeneGm1987
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SpecificityNatural and recombinant mouse CCL21
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity Sample For quantitative detection of mouse CCL21 in cell culture supernates, serum and plas
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Intended UseMouse Gm1987 ELISA Kit allows for the in vitro quantitative determination of Gm1987 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: Mouse CCL21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL21 has been precoated onto 96-well plates. Standards (E Coli, S24-G133) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL21 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
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