Mouse Cckar ELISA Kit

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  • Alternative name

    Mouse CCK-A receptor ELISA Kit;Mouse CCK-AR ELISA Kit;Mouse cholecystokinin-1 receptor ELISA Kit;Mouse CCK1-R ELISA Kit;Mouse AW106902 ELISA Kit;Mouse cholecystokinin A receptor ELISA Kit;Mouse cholecystokinin receptor type A ELISA Kit;

  • Catalog
    E047533
  • species
    Mouse
  • GeneCckar
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CCKAR. No significant cross-reactivity or interference between Mouse CCKAR and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CCKAR and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Cckar ELISA Kit allows for the in vitro quantitative determination of Cckar , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCKAR in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCKAR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CCKAR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCKAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CCKAR. You can calculate the concentration of Mouse CCKAR in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Cholecystokinin receptor type A Protein information
  • Uniprot ID CCKAR_MOUSE
  • Uniprot AC O08786; Q8VCC7; Q9DBV6;
  • UniGene Mm.3521;
  • GeneID 12425
  • KEGG mmu:12425;
  • Mouse Cholecystokinin receptor type A Protein SEQUENCE
  • SEQUENCE 436 AA; 48437 MW; EBB5D8453B792F79 CRC64;

    MDVVDSLLMN GSNITPPCEL GLENETLFCL DQPQPSKEWQ SAVQILLYSF

    IFLLSVLGNT LVITVLIRNK RMRTVTNIFL LSLAVSDLML CLFCMPFNLI

    PNLLKDFIFG SAVCKTTTYF MGTSVSVSTF NLVAISLERY GAICRPLQSR

    VWQTKSHALK VIAATWCLSF TIMTPYPIYS NLVPFTKNNN QTANMCRFLL

    PSDAMQQSWQ TFLLLILFLI PGVVMVVAYG LISLELYQGI KFDASQKKSA

    KEKRLSSGGG GGGGSSSSRY EDSDGCYLQK SRPPRKLELQ QLSTSSSGGR

    INRIRSSGSA ANLIAKKRVI RMLIVIVVLF FLCWMPIFSA NAWRAYDTVS

    AEKHLSGTPI SFILLLSYTS SCVNPIIYCF MNKRFRLGFM ATFPCCPNPG

    PTGVRGEVGE EEDGRTIRAS LSRYSYSHMS TSAPPH

  • UCSC uc008xlm.2; mouse.;



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