SpecificityThis assay has high sensitivity and excellent specificity for detection of CNRIP1. No significant cross-reactivity or interference between CNRIP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNRIP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse Cnrip1 ELISA Kit allows for the in vitro quantitative determination of Cnrip1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description specificalIntended Uses: This CNRIP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CNRIP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||CNRIP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CNRIP1 antibody and an CNRIP1-HRP conjugate. The assay sample and buffer are incubated together with CNRIP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNRIP1 concentration since CNRIP1 from samples and CNRIP1-HRP conjugate compete for the anti-CNRIP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNRIP1 from the sample, fewer sites are left to bind CNRIP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNRIP1 concentration in each sample is interpolated from this standard curve.
Mouse CB1 cannabinoid receptor-interacting protein 1 Protein information
Uniprot ID
CNRP1_MOUSE
Uniprot AC
Q5M8N0; Q3TAC7; Q3UFR2;
UniGene
Mm.425666;
GeneID
380686
KEGG
mmu:380686;
Mouse CB1 cannabinoid receptor-interacting protein 1 Protein SEQUENCE