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Alternative name
Cathepsin L1 ELISA KIT; Cathepsin L ELISA KIT; Major excreted protein ELISA KIT; MEP ELISA KIT; p39 cysteine proteinaseCleaved into the following 2 chains:Cathepsin L1 heavy chain ELISA KIT; Cathepsin L1 light chainCtsl ELISA KIT; Ctsl1 ELISA KIT; MEP ELISA KIT
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Catalog
E047453
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species
Mouse
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GeneCTSL1
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Other Species
Human CTSL1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse CTSL1. No significant cross-reactivity or interference between mouse CTSL1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse CTSL1 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseMouse CTSL1 ELISA Kit allows for the in vitro quantitative determination of CTSL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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