Human B4GALT5 ELISA Kit

Alternative name
Human beta-1,4-GalTase 5 ELISA Kit;Human beta4Gal-T5 ELISA Kit;Human B4Gal-T5 ELISA Kit;Human beta-1,4-GalT II ELISA Kit;Human UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5 ELISA Kit;Human UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5 ELISA Kit;Human BETA4-GALT-IV ELISA Kit;Human beta4GalT-V ELISA Kit;Human gt-V ELISA Kit;Human beta-1,4-galactosyltransferase 5 ELISA Kit;Human UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 ELISA Kit;Human beta-1,4-GalT IV ELISA Kit;Human beta-1.4-galactosyltransferase V ELISA Kit;Human beta4-GalT IV ELISA Kit;
Catalog
E004739
species
Human
GeneB4GALT5
Standard Curve
Other Species Mouse B4galt5 ELISA Kit
SpecificityThis assay recognizes recombinant and natural Human b4Gal-T5. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman B4GALT5 ELISA Kit allows for the in vitro quantitative determination of B4GALT5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to b4Gal-T5, During the reaction, b4Gal-T5 in the sample or standard competes with a fixed amount of biotin-labeled b4Gal-T5 for sites on a pre-coated Monoclonal antibody specific to b4Gal-T5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of b4Gal-T5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Beta-1,4-galactosyltransferase 5 Protein information
Uniprot ID B4GT5_HUMAN
Uniprot AC O43286; E1P625; Q2M394; Q9UJQ8;
UniGene Hs.370487;
GeneID 9334
KEGG hsa:9334;
Human Beta-1,4-galactosyltransferase 5 Protein SEQUENCE
SEQUENCE 388 AA; 45119 MW; A71F3F8999B6E13A CRC64;
MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMMQAQG
ILIRDNVRTI GAQVYEQVLR SAYAKRNSSV NDSDYPLDLN HSETFLQTTT
FLPEDFTYFA NHTCPERLPS MKGPIDINMS EIGMDYIHEL FSKDPTIKLG
GHWKPSDCMP RWKVAILIPF RNRHEHLPVL FRHLLPMLQR QRLQFAFYVV
EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ
MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED
DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER
QGLDGLNNLN YFANITYDAL YKNITVNLTP ELAQVNEY
UCSC uc002xuu.5; human.;

Category

Human B4GALT5 ELISA Kit