Mouse Casp8 ELISA Kit

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  • Alternative name

    Mouse CASP-8 ELISA Kit;Mouse FLICE ELISA Kit;Mouse MACH ELISA Kit;Mouse Mch5 ELISA Kit;Mouse caspase 8 ELISA Kit;Mouse caspase-8 ELISA Kit;Mouse Fas-linked ICE-like protease ELISA Kit;

  • Catalog
    E047238
  • species
    Mouse
  • GeneCasp8
  • Other Species Human CASP8 ELISA KitRat Casp8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CASP8. No significant cross-reactivity or interference between Mouse CASP8 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CASP8 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Casp8 ELISA Kit allows for the in vitro quantitative determination of Casp8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP8 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP8. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CASP8. You can calculate the concentration of Mouse CASP8 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Caspase-8 subunit p10 Protein information
  • Uniprot ID CASP8_MOUSE
  • Uniprot AC QGSNFQKG VPDEAGFEQQ NHTLEVDSSS HKNYIPDEAD FLLGMATVKN CVSYRDPVNG
  • UniGene Mm.336851;
  • GeneID 12370
  • KEGG mmu:12370;
  • Mouse Caspase-8 subunit p10 Protein SEQUENCE
  • SEQUENCE 480 AA; 55357 MW; 045268AE3DE5ED4F CRC64;

    MDFQSCLYAI AEELGSEDLA ALKFLCLDYI PHKKQETIED AQKLFLRLRE

    KGMLEEGNLS FLKELLFHIS RWDLLVNFLD CNREEMVREL RDPDNAQISP

    YRVMLFKLSE EVSELELRSF KFLLNNEIPK CKLEDDLSLL EIFVEMEKRT

    MLAENNLETL KSICDQVNKS LLGKIEDYER SSTERRMSLE GREELPPSVL

    DEMSLKMAEL CDSPREQDSE SRTSDKVYQM KNKPRGYCLI INNHDFSKAR

    EDITQLRKMK DRKGTDCDKE ALSKTFKELH FEIVSYDDCT ANEIHEILEG

    YQSADHKNKD CFICCILSHG DKGVVYGTDG KEASIYDLTS YFTGSKCPSL

    SGKPKIFFIQ ACQGSNFQKG VPDEAGFEQQ NHTLEVDSSS HKNYIPDEAD

    FLLGMATVKN CVSYRDPVNG TWYIQSLCQS LRERCPQGDD ILSILTGVNY

    DVSNKDDRRN KGKQMPQPTF TLRKKLFFPP

  • UCSC uc007bcs.1; mouse.;



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