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Mouse Casp14 ELISA Kit

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  • Alternative name

    Mouse CASP-14 ELISA Kit;Mouse mini-ICE ELISA Kit;Mouse MICE ELISA Kit;Mouse caspase 14 ELISA Kit;Mouse caspase-14 ELISA Kit;
  • Catalog
    E047233
  • species
    Mouse
  • GeneCasp14
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CASP14. No significant cross-reactivity or interference between Mouse CASP14 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CASP14 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseMouse Casp14 ELISA Kit allows for the in vitro quantitative determination of Casp14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP14 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP14. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CASP14. You can calculate the concentration of Mouse CASP14 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Caspase-14 subunit p8, intermediate form Protein information
  • Uniprot ID CASPE_MOUSE
  • Uniprot AC O89094;
  • UniGene Mm.20940;
  • GeneID 12365
  • KEGG mmu:12365;
  • Mouse Caspase-14 subunit p8, intermediate form Protein SEQUENCE

  • SEQUENCE 257 AA; 29458 MW; A228D88DFBA0EB84 CRC64;

    MESEMSDPQP LQEERYDMSG ARLALTLCVT KAREGSEVDM EALERMFRYL

    KFESTMKRDP TAQQFLEELD EFQQTIDNWE EPVSCAFVVL MAHGEEGLLK

    GEDEKMVRLE DLFEVLNNKN CKALRGKPKV YIIQACRGEH RDPGEELRGN

    EELGGDEELG GDEVAVLKNN PQSIPTYTDT LHIYSTVEGY LSYRHDEKGS

    GFIQTLTDVF IHKKGSILEL TEEITRLMAN TEVMQEGKPR KVNPEVQSTL

    RKKLYLQ

  • UCSC uc007fyd.2; mouse. [O89094-1];



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