Human TUBB ELISA Kit

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  • Alternative name

    Human tubulin beta-5 chain ELISA Kit;Human TUBB5 ELISA Kit;Human CDCBM6 ELISA Kit;Human CSCSC1 ELISA Kit;Human M40 ELISA Kit;Human OK/SW-cl.56 ELISA Kit;Human TUBB1 ELISA Kit;Human tubulin beta class I ELISA Kit;Human tubulin beta chain ELISA Kit;Human beta Ib tubulin ELISA Kit;Human tubulin beta-1 chain ELISA Kit;Human tubulin, beta polypeptide ELISA Kit;

  • Catalog
    E004719
  • species
    Human
  • GeneTUBB
  • Standard CurveHuman TUBB ELISA Kit
  • Other Species Mouse TUBB ELISA KitPorcine TUBB ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman TUBB ELISA Kit allows for the in vitro quantitative determination of TUBB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: TUBbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TUBbeta antibody and an TUBbeta-HRP conjugate. The assay sample and buffer are incubated together with TUBbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TUBbeta concentration since TUBbeta from samples and TUBbeta-HRP conjugate compete for the anti-TUBbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by TUBbeta from the sample, fewer sites are left to bind TUBbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TUBbeta concentration in each sample is interpolated from this standard curve.
  • Human Tubulin beta chain Protein information
  • Uniprot ID TBB5_HUMAN
  • Uniprot AC P07437; P05218; Q8WUC1; Q9CY33;
  • UniGene Hs.636480;
  • GeneID 203068
  • KEGG hsa:203068;
  • Human Tubulin beta chain Protein SEQUENCE
  • SEQUENCE 444 AA; 49671 MW; 1E6CD0A36773A103 CRC64;

    MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLDRISVY

    YNEATGGKYV PRAILVDLEP GTMDSVRSGP FGQIFRPDNF VFGQSGAGNN

    WAKGHYTEGA ELVDSVLDVV RKEAESCDCL QGFQLTHSLG GGTGSGMGTL

    LISKIREEYP DRIMNTFSVV PSPKVSDTVV EPYNATLSVH QLVENTDETY

    CIDNEALYDI CFRTLKLTTP TYGDLNHLVS ATMSGVTTCL RFPGQLNADL

    RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVPELT QQVFDAKNMM

    AACDPRHGRY LTVAAVFRGR MSMKEVDEQM LNVQNKNSSY FVEWIPNNVK

    TAVCDIPPRG LKMAVTFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG

    EGMDEMEFTE AESNMNDLVS EYQQYQDATA EEEEDFGEEA EEEA



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