Human BACE2 ELISA Kit

Alternative name
Human Aspartic-like protease 56 kDa ELISA Kit;Human aspartyl protease 1 ELISA Kit;Human ASP1 ELISA Kit;Human Asp 1 ELISA Kit;Human Beta-site amyloid precursor protein cleaving enzyme 2 ELISA Kit;Human Beta-site APP cleaving enzyme 2 ELISA Kit;Human Down region aspartic protease ELISA Kit;Human DRAP ELISA Kit;Human memapsin-1 ELISA Kit;Human membrane-associated aspartic protease 1 ELISA Kit;Human theta-secretase ELISA Kit;Human AEPLC ELISA Kit;Human ALP56 ELISA Kit;Human ASP21 ELISA Kit;Human BAE2 ELISA Kit;Human CDA13 ELISA Kit;Human CEAP1 ELISA Kit;Human beta-site APP-cleaving enzyme 2 ELISA Kit;Human beta-secretase 2 ELISA Kit;Human 56 kDa aspartic-like protease ELISA Kit;Human Down syndrome region aspartic protease ELISA Kit;Human beta-site amyloid beta A4 precursor protein-cleaving enzyme 2 ELISA Kit;Human transmembrane aspartic proteinase Asp1 ELISA Kit;
Catalog
E004711
species
Human
GeneBACE2
Standard Curve
Other Species Mouse Bace2 ELISA Kit Rat Bace2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of BACE2. No significant cross-reactivity or interference between BACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BACE2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman BACE2 ELISA Kit allows for the in vitro quantitative determination of BACE2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalPrinciple of the assay: BACE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BACE2 antibody and an BACE2-HRP conjugate. The assay sample and buffer are incubated together with BACE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BACE2 concentration since BACE2 from samples and BACE2-HRP conjugate compete for the anti-BACE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BACE2 from the sample, fewer sites are left to bind BACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BACE2 concentration in each sample is interpolated from this standard curve.
Human Beta-secretase 2 Protein information
Uniprot ID BACE2_HUMAN
Uniprot AC Q9Y5Z0; A8K7P1; Q5DIH8; Q8N2D4; Q9H2V8; Q9NZL1; Q9NZL2; Q9UJT6;
UniGene Hs.43047; Hs.529408;
GeneID 25825
KEGG hsa:25825;
Human Beta-secretase 2 Protein SEQUENCE
SEQUENCE 518 AA; 56180 MW; 2E903150823760D3 CRC64;
MGALARALLL PLLAQWLLRA APELAPAPFT LPLRVAAATN RVVAPTPGPG
TPAERHADGL ALALEPALAS PAGAANFLAM VDNLQGDSGR GYYLEMLIGT
PPQKLQILVD TGSSNFAVAG TPHSYIDTYF DTERSSTYRS KGFDVTVKYT
QGSWTGFVGE DLVTIPKGFN TSFLVNIATI FESENFFLPG IKWNGILGLA
YATLAKPSSS LETFFDSLVT QANIPNVFSM QMCGAGLPVA GSGTNGGSLV
LGGIEPSLYK GDIWYTPIKE EWYYQIEILK LEIGGQSLNL DCREYNADKA
IVDSGTTLLR LPQKVFDAVV EAVARASLIP EFSDGFWTGS QLACWTNSET
PWSYFPKISI YLRDENSSRS FRITILPQLY IQPMMGAGLN YECYRFGISP
STNALVIGAT VMEGFYVIFD RAQKRVGFAA SPCAEIAGAA VSEISGPFST
EDVASNCVPA QSLSEPILWI VSYALMSVCG AILLVLIVLL LLPFRCQRRP
RDPEVVNDES SLVRHRWK
UCSC uc002yyw.5; human. [Q9Y5Z0-1];

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Human BACE2 ELISA Kit