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Catalog
E004696
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species
Human
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SpecificityNo cross reactivity with other cytokines.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 2 mIU/mL
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Intended UseHuman ST6GAL2 ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyEndocrinology ; Hormones; Research Assays; Assay Categories
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Product Description
specificalPrinciple of the assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-beta HCG monoclonal antibody is pre-coated onto 96-well plates. HRP conjugated anti-beta HCG polyclonal antibody is used as the detection antibody. The standards, test samples, and HRP conjugated detection antibody are added to the wells subsequently, followed by a wash step using prepared wash buffer. TMB substrates are used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the beta HCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of beta HCG can be calculated.
Background: Human chorionic gonadotropin (CG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. It is a glycoprotein composed of 237 amino acids with a molecular mass of 25.7 kDa. It is heterodimeric, with an alpha (alpha) subunit identical to that of luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and beta (beta) subunit that is unique to hCG. beta subunit is secreted by some cancers including seminoma, choriocarcinoma, germ cell tumors, hydatidiform mole formation, teratoma with elements of choriocarcinoma, and islet cell tumor. Combined with alpha-fetoprotein, beta-HCG is an excellent tumor marker for the monitoring of germ cell tumors.
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