-
Catalog
E046919
-
species
Mouse
-
GeneCDT
-
Other Species
Human CDT ELISA Kit
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.78ng/ml
-
Intended UseMouse CDT ELISA Kit allows for the in vitro quantitative determination of CDT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CDT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CDT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CDT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Related products with Mouse CDT ELISA Kit