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Human ENO3 ELISA Kit

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  • Alternative name

    Human 2-phospho-D-glycerate hydro-lyase ELISA Kit;Human enolase 3 ELISA Kit;Human muscle-specific enolase ELISA Kit;Human MSE ELISA Kit;Human skeletal muscle enolase ELISA Kit;Human GSD13 ELISA Kit;Human beta-enolase ELISA Kit;Human 2-phospho-D-glycerate hydrolyase ELISA Kit;Human enolase 3 (beta, muscle) ELISA Kit;Human muscle enriched enolase ELISA Kit;
  • Catalog
    E004690
  • species
    Human
  • GeneENO3
  • Standard CurveHuman ENO3 ELISA Kit
  • Other Species Mouse Eno3 ELISA KitBovine ENO3 ELISA KitChicken ENO3 ELISA KitPorcine ENO3 ELISA KitRabbit ENO3 ELISA KitRat Eno3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ENO3. No significant cross-reactivity or interference between ENO3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ENO3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ENO3 ELISA Kit allows for the in vitro quantitative determination of ENO3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Principle of the assay: ENO3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ENO3 antibody and an ENO3-HRP conjugate. The assay sample and buffer are incubated together with ENO3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ENO3 concentration since ENO3 from samples and ENO3-HRP conjugate compete for the anti-ENO3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ENO3 from the sample, fewer sites are left to bind ENO3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ENO3 concentration in each sample is interpolated from this standard curve.
  • Human Beta-enolase Protein information
  • Uniprot ID ENOB_HUMAN
  • Uniprot AC P13929; B4DUI6; B4DUM6; D3DTL2; E7ENK8; Q96AE2;
  • UniGene Hs.224171;
  • GeneID 2027
  • KEGG hsa:2027;
  • Human Beta-enolase Protein SEQUENCE

  • SEQUENCE 434 AA; 46987 MW; 4D9D8DCF32CF153F CRC64;

    MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR

    DGDKGRYLGK GVLKAVENIN NTLGPALLQK KLSVVDQEKV DKFMIELDGT

    ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF

    NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY

    GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE

    FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD

    WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV

    TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR

    SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK

  • UCSC uc002gac.5; human. [P13929-1];


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