Mouse Pde1c ELISA Kit

Alternative name
Mouse cam-PDE 1C ELISA Kit;Mouse phosphodiesterase 1C ELISA Kit;Mouse calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C ELISA Kit;
Catalog
E046654
species
Mouse
GenePde1c
Other Species Human PDE1C ELISA Kit Rat Pde1c ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseMouse Pde1c ELISA Kit allows for the in vitro quantitative determination of Pde1c , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Pde1c antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Pde1c antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Pde1c amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Pde1c can be calculated.
Mouse Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C Protein information
Uniprot ID PDE1C_MOUSE
Uniprot AC Q64338; Q62045; Q8BSV6;
UniGene Mm.5145;
GeneID 18575
KEGG mmu:18575;
Mouse Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C Protein SEQUENCE
SEQUENCE 706 AA; 80290 MW; 1F58533E1259801F CRC64;
MESPTKEIEE FESNSLKHLQ PEQIEKIWLR LRGLRKYKKT SQRLRSLVKQ
LERGEASVVD LKKNLEYAAT VLESVYIDET RRLLDTEDEL SDIQSDAVPS
EVRDWLASTF TRQMGMMLRR SDEKPRFKSI VHAVQAGIFV ERMYRRTSNM
VGLSYPPAVI DALKDVDTWS FDVFSLNEAS GDHALKFIFY ELLTRYDLIS
RFKIPISALV SFVEALEVGY SKHKNPYHNL MHAADVTQTV HYLLYKTGVA
NWLTELEIFA IIFSAAIHDY EHTGTTNNFH IQTRSDPAIL YNDRSVLENH
HLSAAYRLLQ EDEEMNILVN LSKDDWREFR TLVIEMVMAT DMSCHFQQIK
AMKTALQQPE AIEKPKALSL MLHTADISHP AKAWDLHHRW TMSLLEEFFR
QGDREAELGL PFSPLCDRKS TMVAQSQVGF IDFIVEPTFT VLTDMTEKIV
SPLIDESSQT GGTGQRRSSL NSINSSDAKR SGVKSSGSDG SAPINNSVIP
VDYKSFKATW TEVVQINRER WRAKVPKEEK AKKEAEEKAR LAAEEKQKEM
EAKSQAEQGT TSKGEKKTSG EAKSQVNGTR KGDNPRGKNS KGEKAGEKQQ
NGDLKDGKNK ADKKDHSNTG NESKKTDGTK KRSHGSPAPS TSSTSRITLP
VIKPPLRHFK RPAYASSSYA PSVPKKTDDH PVRYKMLDQR IKMKKIQNIS
HHWNKK
UCSC uc009cbb.2; mouse. [Q64338-1];

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Category

Mouse Pde1c ELISA Kit

Mouse cam-PDE 1C ELISA Kit;Mouse phosphodiesterase 1C ELISA Kit;Mouse calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C ELISA Kit;