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Alternative name
Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APPI ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid protein ELISA KIT; Cerebral vascular amyloid peptide ELISA KIT; CVAP ELISA KIT; PreA4 ELISA KIT; Protease nexin-II ELISA KIT; PN-IIAPP ELISA KIT; A4 ELISA KIT; AD1 ELISA KIT; APP ELISA KIT; CVAP ELISA KIT; PN-II ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT
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Catalog
E004661
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species
Human
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Genebeta APP
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Standard Curve
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Other Species
Human beta-APP ELISA KitMouse beta-APP ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman beta APP ELISA Kit allows for the in vitro quantitative determination of beta APP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyNeurobiology
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Product Description
specificalPrinciple of the Assay: beta-APP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for beta-APP. Standards or samples are then added to the microtiter plate wells and beta-APP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of beta-APP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for beta-APP are added to each well to "sandwich" the beta-APP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain beta-APP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The beta-APP concentration in each sample is interpolated from this standard curve.
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