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Mouse Casr ELISA Kit

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  • Alternative name

    Mouse CaSR ELISA Kit;Mouse parathyroid cell calcium-sensing receptor ELISA Kit;Mouse PCaR1 ELISA Kit;Mouse Gprc2a ELISA Kit;Mouse CaR ELISA Kit;Mouse calcium-sensing receptor ELISA Kit;Mouse extracellular calcium-sensing receptor ELISA Kit;Mouse G protein-coupled receptor, family C, group 2, member A ELISA Kit;Mouse cation sensing receptor ELISA Kit;
  • Catalog
    E046608
  • species
    Mouse
  • GeneCasr
  • Standard CurveMouse Casr ELISA Kit
  • Other Species Human CASR ELISA KitPorcine CASR ELISA KitRat Casr ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CASR. No significant cross-reactivity or interference between CASR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CASR and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Casr ELISA Kit allows for the in vitro quantitative determination of Casr , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: CASR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CASR antibody and an CASR-HRP conjugate. The assay sample and buffer are incubated together with CASR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASR concentration since CASR from samples and CASR-HRP conjugate compete for the anti-CASR antibody binding site. Since the number of sites is limited, as more sites are occupied by CASR from the sample, fewer sites are left to bind CASR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASR concentration in each sample is interpolated from this standard curve.
  • Mouse Extracellular calcium-sensing receptor Protein information
  • Uniprot ID CASR_MOUSE
  • Uniprot AC Q9QY96; G3UX06; O08968; O88519; Q9QY95; Q9QZU8; Q9R1D6; Q9R1Y2;
  • UniGene Mm.103619; Mm.393142;
  • GeneID 12374
  • KEGG mmu:12374;
  • Mouse Extracellular calcium-sensing receptor Protein SEQUENCE

  • SEQUENCE 1079 AA; 120903 MW; 8AEF586CDCD93CB3 CRC64;

    MAWFGYCLAL LALTWHSSAY GPDQRAQKKG DIILGGLFPI HFGVAAKDQD

    LKSRPESVEC IRYNFRGFRW LQAMIFAIEE INSSPALLPN MTLGYRIFDT

    CNTVSKALEA TLSFVAQNKI DSLNLDEFCN CSEHIPSTIA VVGATGSGVS

    TAVANLLGLF YIPQVSYASS SRLLSNKNQF KSFLRTIPND EHQATAMADI

    IEYFRWNWVG TIAADDDYGR PGIEKFREEA EERDICIDFS ELISQYSDEE

    EIQQVVEVIQ NSTAKVIVVF SSGPDLEPLI KEIVRRNITG RIWLASEAWA

    SSSLIAMPEY FHVVGGTIGF GLKAGQIPGF REFLQKVHPR KSVHNGFAKE

    FWEETFNCHL QDGAKGPLPV DTFVRSHEEG GNRLLNSSTA FRPLCTGDEN

    INSVETPYMD YEHLRISYNV YLAVYSIAHA LQDIYTCLPG RGLFTNGSCA

    DIKKVEAWQV LKHLRHLNFT NNMGEQVTFD ECGDLVGNYS IINWHLSPED

    GSIVFKEVGY YNVYAKKGER LFINEGKILW SGFSREVPFS NCSRDCQAGT

    RKGIIEGEPT CCFECVECPD GEYSGETDAS ACDKCPDDFW SNENHTSCIA

    KEIEFLAWTE PFGIALTLFA VLGIFLTAFV LGVFIKFRNT PIVKATNREL

    SYLLLFSLLC CFSSSLFFIG EPQDWTCRLR QPAFGISFVL CISCILVKTN

    RVLLVFEAKI PTSFHRKWWG LNLQFLLVFL CTFMQIVICI IWLYTAPPSS

    YRNHELEDEI IFITCHEGSL MALGSLIGYT CLLAAICFFF AFKSRKLPEN

    FNEAKFITFS MLIFFIVWIS FIPAYASTYG KFVSAVEVIA ILAASFGLLA

    CIFFNKVYII LFKPSRNTIE EVRSSTAAHA FKVAARATLR RPNISRKRSS

    SLGGSTGSIP SSSISSKSNS EDRFPQPERQ KQQQPLALTQ QEQQQQPLTL

    QPQQQQQPQQ PRCKQKVIFG SGTVTFSLSF DEPQKNAMAH RNSMRQNSLE

    AQKSNDTLNR HQALLPLQCA EADSEMTIQE TGLQGPMVGD HQPEIESPDE

    MSPALVMSTS RSFVISGGGS SVTENILHS

  • UCSC uc012afg.1; mouse. [Q9QY96-2];


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