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Mouse Cacna1f ELISA Kit

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  • Alternative name

    Mouse Voltage-gated calcium channel subunit alpha Cav1.4 ELISA Kit;
  • Catalog
    E046579
  • species
    Mouse
  • GeneCacna1f
  • Standard CurveMouse Cacna1f ELISA Kit
  • Other Species Human CACNA1F ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.106ng/mL.
  • Intended UseMouse Cacna1f ELISA Kit allows for the in vitro quantitative determination of Cacna1f , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Voltage-dependent L-type calcium channel subunit alpha-1F Protein information
  • Uniprot ID CAC1F_MOUSE
  • Uniprot AC Q9JIS7;
  • UniGene Mm.289647;
  • Mouse Voltage-dependent L-type calcium channel subunit alpha-1F Protein SEQUENCE

  • SEQUENCE 1985 AA; 221926 MW; C365A105614ACAD5 CRC64;

    MSESEVGKDT TPEPSPANGT GPGPEWGLCP GPPTVGTDTS GASGLGTPRR

    RTQHNKHKTV AVASAQRSPR ALFCLTLTNP IRRSCISIVE WKPFDILILL

    TIFANCVALG VYIPFPEDDS NTANHNLEQV EYVFLVIFTV ETVLKIVAYG

    LVLHPSAYIR NGWNLLDFII VVVGLFSVLL EQGPGRPGDA PHTGGKPGGF

    DVKALRAFRV LRPLRLVSGV PSLHIVVNSI MKALVPLLHI ALLVLFVIII

    YAIIGLELFL GRMHKTCYFL GSDMEAEEDP SPCASSGSGR SCTLNHTECR

    GRWPGPNGGI TNFDNFFFAM LTVFQCITME GWTDVLYWMQ DAMGYELPWV

    YFVSLVIFGS FFVLNLVLGV LSGEFSKERE KAKARGDFQK LREKQQMEED

    LRGYLDWITQ AEELDLHDPS VDGNLASLAE EGRAGHRPQL SELTNRRRGR

    LRWFSHSTRS THSTSSHASL PASDTGSMTD TPGDEDEEEG TMASCTRCLN

    KIMKTRICRH FRRANRGLRA RCRRAVKSNA CYWAVLLLVF LNTLTIASEH

    HGQPLWLTQT QEYANKVLLC LFTVEMLLKL YGLGPSVYVA SFFNRFDCFV

    VCGGILETTL VEVGAMQPLG ISVLRCVRLL RIFKVTRHWA SLSNLVASLL

    NSMKSIASLL LLLFLFIIIF SLLGMQLFGG KFNFDQTHTK RSTFDTFPQA

    LLTVFQILTG EDWNVVMYDG IMAYGGPFFP GMLVCVYFII LFICGNYILL

    NVFLAIAVDN LASGDAGTAK DKGREKSSEG NPPKENKVLV PGGENEDAKG

    ARSEGAAPGM EEEEEEEEEE EEEEEEEEEN GAGHVELLQE VVPKEKVVPI

    PEGSAFFCLS QTNPLRKACH TLIHHHIFTS LILVFIILSS VSLAAEDPIR

    AHSFRNHILG YFDYAFTSIF TVEILLKMTV FGAFLHRGSF CRSWFNLLDL

    LVVSVSLISF GIHSSAISVV KILRVLRVLR PLRAINRAKG LKHVVQCVFV

    AIRTIGNIMI VTTLLQFMFA CIGVQLFKGK FYSCTDEAKH TLKESKGSFL

    IYPDGDVSRP LVRERLWVNS DFNFDNVLSA MMALFTVSTF EGWPALLYKA

    IDANAEDEGP IYNYHVEISV FFIVYIIIIA FFMMNIFVGF VIITFRAQGE

    QEYQNCELDK NQRQCVEYAL KAQPLRRYIP KNPHQYRVWA TVNSRAFEYL

    MFLLILLNTV ALAMQHYEQT APFNYAMDIL NMVFTGLFTI EMVLKIIAFK

    PKHYFADAWN TFDALIVVGS VVDIAVTEVN NGGHLGESSE DTSRISITFF

    RLFRVMRLVK LLSKGEGIRT LLWTFIKSFQ ALPYVALLIA MIFFIYAVIG

    MQMFGLVALQ DGTQINRNNN FQTFPQAVLL LFRCATGEAW QEIMLASLPG

    NRCDPESDFG PGEEFTCGSS FAIVYFISFF MLCAFLIINL FVAVIMDNFD

    YLTRDWSILG PHHLDEFKRI WSEYDPGAKG RIKHLDVVAL LRRIQPPLGF

    GKLCPHRVAC KRLVAMNVPL NSDGTVTFNA TLFALVRTSL KIKTEGNLDQ

    ANQELRMVIK KIWKRIKQKL LDEVIPPPDE EEVTVGKFYA TFLIQDYFRK

    FRRRKEKGLL GREAPTSTSS ALQAGLRSLQ DLGPEIRQAL TYVTEEEEEE

    EEAVGQEAEE EEAENNPEPY KDSIDSQPQS RWNSRISVSL PVKEKLPDSL

    STGPSDDDGL APNSRQPSVI QAGSQPHRRS SGVFMFTIPE EGSIQLKGTQ

    GQDNQNEEQE LPDWTPDLDR AGRDSFEPSP FTTSLVQQHV NGHMSTPTFA

    APHACRSEPS FTIQCLQRLG SCEDLPIPGT YHRGRTSGPS RAQGSWAAPP

    QKGRLLYAPL LLVEESTVGE GYLGKLGGPL RTFTCLQVPG AHPNPSHRKR

    GSADSLVEAV LISEGLGLFA QDPRFVALAK QEIADACHLT LDEMDSAASD

    LLAQRTISLY SDEESILSRF DEEDLGDEMA CVHAL




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