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Mouse Clec12a ELISA Kit

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  • Alternative name

    Mouse C-type lectin-like molecule 1 ELISA Kit;Mouse CLL-1 ELISA Kit;Mouse myeloid inhibitory C-type lectin-like receptor ELISA Kit;Mouse Micl ELISA Kit;Mouse D230024O04 ELISA Kit;Mouse KLRL1 ELISA Kit;Mouse C-type lectin domain family 12, member a ELISA Kit;Mouse C-type lectin domain family 12 member A ELISA Kit;
  • Catalog
    E046339
  • species
    Mouse
  • GeneClec12a
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CLEC12A. No significant cross-reactivity or interference between CLEC12A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC12A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Clec12a ELISA Kit allows for the in vitro quantitative determination of Clec12a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the assay: CLEC12A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC12A antibody and an CLEC12A-HRP conjugate. The assay sample and buffer are incubated together with CLEC12A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC12A concentration since CLEC12A from samples and CLEC12A-HRP conjugate compete for the anti-CLEC12A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC12A from the sample, fewer sites are left to bind CLEC12A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC12A concentration in each sample is interpolated from this standard curve.
  • Mouse C-type lectin domain family 12 member A Protein information
  • Uniprot ID CL12A_MOUSE
  • Uniprot AC Q504P2; Q8BN96;
  • UniGene Mm.90241;
  • GeneID 232413
  • KEGG mmu:232413;
  • Mouse C-type lectin domain family 12 member A Protein SEQUENCE

  • SEQUENCE 267 AA; 30757 MW; 2958572936E4A35D CRC64;

    MSEEIVYANL KIQDPDKKEE TQKSDKCGGK VSADASHSQQ KTVLILILLC

    LLLFIGMGVL GGIFYTTLAT EMIKSNQLQR AKEELQENVF LQLKHNLNSS

    KKIKNLSAML QSTATQLCRE LYSKEPEHKC KPCPKGSEWY KDSCYSQLNQ

    YGTWQESVMA CSARNASLLK VKNKDVLEFI KYKKLRYFWL ALLPRKDRTQ

    YPLSEKMFLS EESERSTDDI DKKYCGYIDR VNVYYTYCTD ENNIICEETA

    SKVQLESVLN GLPEDSR

  • UCSC uc009efl.2; mouse.;



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