Mouse Ccr9 ELISA Kit

Alternative name
Mouse C-C CKR-9 ELISA Kit;Mouse CC-CKR-9 ELISA Kit;Mouse CCR-9 ELISA Kit;Mouse chemokine C-C receptor 10 ELISA Kit;Mouse Cmkbr10 ELISA Kit;Mouse A130091K22Rik ELISA Kit;Mouse GPR-9-6 ELISA Kit;Mouse chemokine (C-C motif) receptor 9 ELISA Kit;Mouse C-C chemokine receptor type 9 ELISA Kit;
Catalog
E046252
species
Mouse
GeneCcr9
Standard Curve
Other Species Human CCR9 ELISA Kit Sheep CCR9 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCR-9. No significant cross-reactivity or interference between CCR-9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCR-9 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Ccr9 ELISA Kit allows for the in vitro quantitative determination of Ccr9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CCR-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCR-9 antibody and an CCR-9-HRP conjugate. The assay sample and buffer are incubated together with CCR-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCR-9 concentration since CCR-9 from samples and CCR-9-HRP conjugate compete for the anti-CCR-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCR-9 from the sample, fewer sites are left to bind CCR-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCR-9 concentration in each sample is interpolated from this standard curve.
Mouse C-C chemokine receptor type 9 Protein information
Uniprot ID CCR9_MOUSE
Uniprot AC Q9WUT7; A2RSM5; Q543I4;
UniGene Mm.440604; Mm.442383;
GeneID 12769
KEGG mmu:12769;
Mouse C-C chemokine receptor type 9 Protein SEQUENCE
SEQUENCE 369 AA; 41913 MW; 6971F76F0A24B4AE CRC64;
MMPTELTSLI PGMFDDFSYD STASTDDYMN LNFSSFFCKK NNVRQFASHF
LPPLYWLVFI VGTLGNSLVI LVYWYCTRVK TMTDMFLLNL AIADLLFLAT
LPFWAIAAAG QWMFQTFMCK VVNSMYKMNF YSCVLLIMCI SVDRYIAIVQ
AMKAQVWRQK RLLYSKMVCI TIWVMAAVLC TPEILYSQVS GESGIATCTM
VYPKDKNAKL KSAVLILKVT LGFFLPFMVM AFCYTIIIHT LVQAKKSSKH
KALKVTITVL TVFIMSQFPY NSILVVQAVD AYAMFISNCT ISTNIDICFQ
VTQTIAFFHS CLNPVLYVFV GERFRRDLVK TLKNLGCISQ AQWVSFTRRE
GSLKLSSMLL ETTSGALSL
UCSC uc009sgl.2; mouse.;

Category

Mouse Ccr9 ELISA Kit

Mouse C-C CKR-9 ELISA Kit;Mouse CC-CKR-9 ELISA Kit;Mouse CCR-9 ELISA Kit;Mouse chemokine C-C receptor 10 ELISA Kit;Mouse Cmkbr10 ELISA Kit;Mouse A130091K22Rik ELISA Kit;Mouse GPR-9-6 ELISA Kit;Mouse chemokine (C-C motif) receptor 9 ELISA Kit;Mouse C-C chemokine receptor type 9 ELISA Kit;